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Clinical Chemistry 39: 689-692, 1993;
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Clinical Chemistry, Vol 39, 689-692, Copyright © 1993 by American Association for Clinical Chemistry

Quantitative analysis of serum proteins separated by capillary electrophoresis

JW Kim, JH Park, JW Park, HJ Doh, GS Heo and KJ Lee
Department of Clinical Pathology, Chungnam National University Hospital, Daejonsi, Korea.

The possibility of open tubular capillary electrophoresis for clinical diagnostic use is examined. Capillary electrophoresis was performed in an untreated 50 microns (i.d.) x 100 cm (65 cm to detector) capillary with detection of absorbance at 200 nm. Conditions for the separation of serum proteins without adsorption to the capillary surface were established. Quantitative analyses of serum samples from 38 patients with liver cirrhosis, nephrotic syndrome, or polyclonal gammopathy by capillary electrophoresis were done and the results were compared with those by conventional agarose gel electrophoresis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. All samples were analyzed in duplicate. We evaluated linearity of response, within-run CV, and the correlation between capillary electrophoresis and agarose gel electrophoresis.


The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
J. Bienvenu, M. S. Graziani, F. Arpin, H. Bernon, C. Blessum, C. Marchetti, G. Righetti, M. Somenzini, G. Verga, and F. Aguzzi
Multicenter evaluation of the Paragon CZETM 2000 capillary zone electrophoresis system for serum protein electrophoresis and monoclonal component typing
Clin. Chem., March 1, 1998; 44(3): 599 - 605.
[Abstract] [Full Text] [PDF]


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Copyright © 1993 by the American Association for Clinical Chemistry.