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Clinical Chemistry 39: 782-788, 1993;
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Clinical Chemistry, Vol 39, 782-788, Copyright © 1993 by American Association for Clinical Chemistry

Increased phospholipase A activities in sera of intensive-care patients show sn-2 specificity but no acyl-chain selectivity

M Puttmann, J Aufenanger, E von Ochsenstein, S Durholt, K van Ackern, J Harenberg and GE Hoffmann
Institut fur Klinische Chemie, Klinikum Mannheim, Universitat Heidelberg, Germany.

Phospholipase A (PLA) activities were measured by high-performance liquid chromatography in two enzyme preparations purified from human duodenal juice and a serum pool as well as in 52 sera from 31 intensive- care patients with various diseases. On the basis of a position- specific fatty acid analysis of the natural substrate ("soybean lecithin") from a commercial PLA kit, serum activities of PLA1 could be clearly distinguished from those of PLA2, which is not possible in the usual measurements made with single-label radioactive substrates. Independent of the type of disease, all sera with highly increased PLA activities (40-200 U/L) showed nearly pure PLA2 characteristics without any preference among oleic, linoleic, and linolenic acid in the sn-2 position of the glycerophospholipid substrate. Nevertheless, very low PLA1 activities (< or = 5 U/L, most likely due to heparin perfusion therapy) could also be detected by palmitic and stearic acid release from the sn-1 position, leading to small changes in fatty acid release patterns of sera with low PLA activities. Measurements with sera from heparin-treated volunteers demonstrated that heparin therapy may initially contribute as much as 22 U/L to increased PLA1 activities but is not important under prolonged therapy. The absence of selectivity with respect to acyl-chain desaturation supports the concept of serum PLA2 as an acute-phase protein rather than a regulator of the arachidonic acid cascade.


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J. Goyal, K. Wang, M. Liu, and P. V. Subbaiah
Novel Function of Lecithin-Cholesterol Acyltransferase. HYDROLYSIS OF OXIDIZED POLAR PHOSPHOLIPIDS GENERATED DURING LIPOPROTEIN OXIDATION
J. Biol. Chem., June 27, 1997; 272(26): 16231 - 16239.
[Abstract] [Full Text] [PDF]


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Copyright © 1993 by the American Association for Clinical Chemistry.