Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 39: 820-824, 1993;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ou, C. N.
Right arrow Articles by Rognerud, C. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ou, C. N.
Right arrow Articles by Rognerud, C. L.

Clinical Chemistry, Vol 39, 820-824, Copyright © 1993 by American Association for Clinical Chemistry

Rapid analysis of hemoglobin variants by cation-exchange HPLC

CN Ou and CL Rognerud
Department of Pathology, Texas Children's Hospital, Houston 77030.

We investigated the use of a 3.5 x 0.46 cm HPLC column packed with 5- microns particles of porous (100 nm) silica coated with polyaspartic acid for hemoglobin analysis. A 13-min gradient was produced between two mobile phases. The method is capable of separating more than 35 commonly encountered hemoglobin variants within 12 min. Hemoglobin variants identified include Bart's, acetyl F, H, A1c, F, Camden, N- Baltimore, J-Baltimore, N-Seattle, Grady, Fannin-Lubbock, A G-Georgia, Lepore-Baltimore, P-Galveston, G-Coushatta, Lepore-Boston, E, Osu Christiansborg, A2, G-Philadelphia, Korle Bu, Russ, Richmond, D-Los Angeles, Deer Lodge, Montgomery, S, Q-Thailand, G-San Jose, A2', Hasharon, Q-India, Tampa, GS hybrid, C-Harlem, O-Arab, British Columbia, and C. Between-run precision of an in-house pooled hemoglobin control material, AFSCA2, gave CVs of 2-5% for the A, F, S, and C and 8% for the A2 over a 6-month period. The simplicity of sample preparation, high resolution of the system, and high accuracy of the method, combined with complete automation, make this an ideal methodology for the routine diagnosis of hemoglobin disorders in a clinical laboratory.


The following articles in journals at HighWire Press have cited this article:


Home page
 haematolHome page
E. J. van Beers, C. F.J. van Tuijn, M. R. Mac Gillavry, A. van der Giessen, J.-J. B. Schnog, B. J. Biemond, and on behalf of the CURAMA study group
Sickle cell disease-related organ damage occurs irrespective of pain rate: implications for clinical practice
Haematologica, May 1, 2008; 93(5): 757 - 760.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
R. Mabaera, M. R. Greene, C. A. Richardson, S. J. Conine, C. D. Kozul, and C. H. Lowrey
Neither DNA hypomethylation nor changes in the kinetics of erythroid differentiation explain 5-azacytidine's ability to induce human fetal hemoglobin
Blood, January 1, 2008; 111(1): 411 - 420.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
R. Mabaera, C. A. Richardson, K. Johnson, M. Hsu, S. Fiering, and C. H. Lowrey
Developmental- and differentiation-specific patterns of human {gamma}- and {beta}-globin promoter DNA methylation
Blood, August 15, 2007; 110(4): 1343 - 1352.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
A. Joutovsky, J. Hadzi-Nesic, and M. A. Nardi
HPLC Retention Time as a Diagnostic Tool for Hemoglobin Variants and Hemoglobinopathies: A Study of 60000 Samples in a Clinical Diagnostic Laboratory
Clin. Chem., October 1, 2004; 50(10): 1736 - 1747.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
G. M. Clarke and T. N. Higgins
Laboratory Investigation of Hemoglobinopathies and Thalassemias: Review and Update
Clin. Chem., August 1, 2000; 46(8): 1284 - 1290.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
R. M. Bohmer, T. A. Campbell, and D. W. Bianchi
Selectively increased growth of fetal hemoglobin-expressing adult erythroid progenitors after brief treatment of early progenitors with transforming growth factor beta
Blood, May 1, 2000; 95(9): 2967 - 2974.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
N. Mario, B. Baudin, A. Bruneel, J. Janssens, and M. Vaubourdolle
Capillary Zone Electrophoresis for the Diagnosis of Congenital Hemoglobinopathies
Clin. Chem., February 1, 1999; 45(2): 285 - 288.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
A. A. Mohammad, A. O. Okorodudu, M. G. Bissell, P. Dow, G. Reger, A. Meier, P. Guodagno, and J. R. Petersen
Clinical Application of Capillary Isoelectric Focusing on Fused Silica Capillary for Determination of Hemoglobin Variants
Clin. Chem., September 1, 1997; 43(9): 1798 - 1799.
[Full Text]

Home page
 Clin. Chem.Home page
J. Riou, C. Godart, D. Hurtrel, M. Mathis, C. Bimet, J. Bardakdjian-Michau, C. Prehu, H. Wajcman, and F. Galacteros
Cation-exchange HPLC evaluated for presumptive identification of hemoglobin variants
Clin. Chem., January 1, 1997; 43(1): 34 - 39.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1993 by the American Association for Clinical Chemistry.