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Clinical Chemistry 39: 1007-1011, 1993;
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Clinical Chemistry, Vol 39, 1007-1011, Copyright © 1993 by American Association for Clinical Chemistry

Enzymatic determination of anti-NAD+ glycohydrolase in serum

FJ Gella, J Viver, F Canalias, J Beleta and F Gonzalez-Sastre
Department de Bioquimica, Hospital de la Santa Creu i Sant Pau, Universitat Autonoma de Barcelona, Spain.

A colorimetric method for measuring anti-NAD+ glycohydrolase in human sera has been developed. The assay involves the inhibition of NAD+ glycohydrolase (EC 3.2.2.5) by the antibody and determination of the noninhibited enzyme activity by using an enzymatic amplifying system for NAD+. The assay is easily carried out and has the additional advantage of a direct relationship between signal and antibody concentration. The results obtained for 100 human sera compare favorably with other tests commonly used to obtain evidence of streptococcal infections or their complications, such as the anti- streptolysin O and the anti-DNase B tests.




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Copyright © 1993 by the American Association for Clinical Chemistry.