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Clinical Chemistry 39: 1086-1088, 1993;
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Clinical Chemistry, Vol 39, 1086-1088, Copyright © 1993 by American Association for Clinical Chemistry

Commercial kits for 1,25-dihydroxyvitamin D compared with a liquid- chromatographic assay

S Bertelloni, GI Baroncelli, U Benedetti, G Franchi and G Saggese
Department of Pediatrics, University of Pisa, Italy.

Three commercially available kits for 1,25-dihydroxyvitamin D [1,25(OH)2D] determination were compared with a liquid-chromatographic method. Serum samples were analyzed for 1,25(OH)2D from 70 healthy subjects (age 2.2-17.5 years; 35 males, 35 females), some (n = 5) given orally high vitamin D3 doses. In addition, 1,25(OH)2D was measured in 28 patients with untreated diseases associated with low (n = 16) or high (n = 12) serum concentrations of the hormone. The results showed that the commercial kits were sufficiently accurate with respect to the comparison method and suitable for routine clinical work.




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Copyright © 1993 by the American Association for Clinical Chemistry.