Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 39: 1369-1374, 1993;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kao, P. C.
Right arrow Articles by Schryver, P. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kao, P. C.
Right arrow Articles by Schryver, P. G.

Clinical Chemistry, Vol 39, 1369-1374, Copyright © 1993 by American Association for Clinical Chemistry

Development and evaluation of an osteocalcin chemiluminoimmunoassay

PC Kao, BL Riggs and PG Schryver
Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905.

We developed a competitive chemiluminoimmunoassay of osteocalcin that is similar to radioimmunoassay but uses acridinium-ester-labeled antigen instead of 125I-labeled osteocalcin; a second antibody immobilized on plastic beads is used to separate free and bound fractions. There was good correlation of the new chemiluminoimmunoassay (y) with a polyclonal antiserum (R102) radioimmunoassay (x) used in many previous clinical studies (r = 0.96, y = 0.968x + 2.69, Sy/x = 0.029, n = 86). The new assay recognized both the intact and the small fragment of osteocalcin in plasma and detected decreases of them (total, approximately 47%) after a 24-h infusion of parathyroid hormone. Patients with primary hyperparathyroidism had increased concentrations of intact osteocalcin. Children had higher concentrations of osteocalcin than adults did. Healthy women had greater osteocalcin concentrations at ages 50-70 years than earlier. Inverse correlations of bone mineral density and osteocalcin were found in healthy women and in women with osteoporosis.


The following articles in journals at HighWire Press have cited this article:


Home page
 J. Clin. Endocrinol. Metab.Home page
H. M. Heshmati, B. L. Riggs, M. F. Burritt, C. A. McAlister, P. C. Wollan, and S. Khosla
Effects of the Circadian Variation in Serum Cortisol on Markers of Bone Turnover and Calcium Homeostasis in Normal Postmenopausal Women
J. Clin. Endocrinol. Metab., March 1, 1998; 83(3): 751 - 756.
[Abstract] [Full Text]

Home page
 NEJMHome page
Q. Cai, S. F. Hodgson, P. C. Kao, V. A. Lennon, G. G. Klee, A. R. Zinsmiester, and R. Kumar
Inhibition of Renal Phosphate Transport by a Tumor Product in a Patient with Oncogenic Osteomalacia
N. Engl. J. Med., June 9, 1994; 330(23): 1645 - 1649.
[Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1993 by the American Association for Clinical Chemistry.