Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 39: 1439-1443, 1993;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ishimori, Y.
Right arrow Articles by Rokugawa, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ishimori, Y.
Right arrow Articles by Rokugawa, K.

Clinical Chemistry, Vol 39, 1439-1443, Copyright © 1993 by American Association for Clinical Chemistry

Stable liposomes for assays of human sera

Y Ishimori and K Rokugawa
Materials and Devices Research Laboratories, Toshiba Research and Development Center, Kawasaki, Japan.

We report a novel homogeneous immunoassay system involving protein- bearing liposome-encapsulated carboxyfluorescein as a release marker. We applied this system to determine protein antigens, e.g., ferritin, in human serum samples by a sandwich-type assay. Liposomal lysis was observed in many samples, even though no second antibody was added to the reaction mixture. We demonstrated that the functional groups used to immobilize an antibody on liposomes are related to this phenomenon. Stable liposomes in human sera were prepared by incorporating bromoacetyl groups instead of the dithiopyridyl groups used previously. A good correlation (y = 0.98x - 8.81, r = 0.98, Sx/y = 66.9, range approximately 10-2000 micrograms/L) with results by RIA was obtained in the ferritin measurement of 53 patients' sera by using these liposomes.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1993 by the American Association for Clinical Chemistry.