Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 39: 1717-1723, 1993;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Weykamp, C. W.
Right arrow Articles by van der Slik, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Weykamp, C. W.
Right arrow Articles by van der Slik, W.

Clinical Chemistry, Vol 39, 1717-1723, Copyright © 1993 by American Association for Clinical Chemistry

Influence of hemoglobin variants and derivatives on glycohemoglobin determinations, as investigated by 102 laboratories using 16 methods

CW Weykamp, TJ Penders, FA Muskiet and W van der Slik
Department of Clinical Chemistry, Queen Beatrix Hospital, Winterswijk, The Netherlands.

Influences of hemoglobin (Hb) variants (HbSS, HbCC, beta-thalassemia, HbAE, HbAS, HbAC, hereditary persistent HbF) and Hb derivatives (carbamylated- and acetylated-Hbs, Schiff base, and those formed in stored blood) on results of glyco-Hb assays by 102 laboratories using 16 different methods were investigated. Affinity chromatography shows deviating results only with homozygous Hb S and C. Correct interpretation of results from patients with decreased erythrocyte half- lives requires previous knowledge on this condition. Measurements of HbA1c by HPLC and electrophoresis are obviously unsuitable for homozygous hemoglobinopathies; for heterozygous hemoglobinopathies and Hb synthesis variants, HbA1c should be expressed as percentage of HbA0 + HbA1c; abnormal Hbs are usually recognized; both carbamylated- and acetylated-Hbs interfere and Schiff base must be eliminated. Except for stored blood, all Hb variants and derivatives gave erroneous results with disposable ion-exchange columns. Dako's immunoassay is not affected by Hb derivatives; glycated Hb variants are not recognized as glyco-Hb and percentages are consequently too low. Glyco-Hb by the immunoassay of Bayer (performed by one laboratory) is not affected by Hb variants and derivatives.


The following articles in journals at HighWire Press have cited this article:


Home page
 Annals of Clinical & Laboratory ScienceHome page
J. M. Abadie and A. A. Koelsch
Performance of the Roche Second Generation Hemoglobin A1c Immunoassay in the Presence of Hb-S or Hb-C Traits
Ann. Clin. Lab. Sci., January 1, 2008; 38(1): 31 - 36.
[Abstract] [Full Text] [PDF]

Home page
 Diabetes Spectr.Home page
A. Smaldone
Glycemic Control and Hemoglobinopathy: When A1C May Not Be Reliable
Diabetes Spectr, January 1, 2008; 21(1): 46 - 49.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
S.-T. Lee, C. W. Weykamp, Y.-W. Lee, J.-W. Kim, and C.-S. Ki
Effects of 7 Hemoglobin Variants on the Measurement of Glycohemoglobin by 14 Analytical Methods
Clin. Chem., December 1, 2007; 53(12): 2202 - 2205.
[Abstract] [Full Text] [PDF]

Home page
 Diabetes CareHome page
D. J. Tascona, A. R. Morton, E. B. Toffelmire, D. C. Holland, and E. A. Iliescu
Adequacy of Glycemic Control in Hemodialysis Patients With Diabetes
Diabetes Care, October 1, 2006; 29(10): 2247 - 2251.
[Abstract] [Full Text] [PDF]

Home page
 Diabetes CareHome page
D. E. Goldstein, R. R. Little, R. A. Lorenz, J. I. Malone, D. Nathan, C. M. Peterson, and D. B. Sacks
Tests of Glycemia in Diabetes
Diabetes Care, July 1, 2004; 27(7): 1761 - 1773.
[Full Text] [PDF]

Home page
 The Annals of PharmacotherapyHome page
B. L Armor and M. L Britton
Diabetes Mellitus Non-Glucose Monitoring: Point-of-Care Testing
Ann. Pharmacother., June 1, 2004; 38(6): 1039 - 1047.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Pathol.Home page
J L Camargo and J L Gross
Conditions associated with very low values of glycohaemoglobin measured by an HPLC method
J. Clin. Pathol., April 1, 2004; 57(4): 346 - 349.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
D. B. Sacks
Hemoglobin Variants and Hemoglobin A1c Analysis: Problem Solved?
Clin. Chem., August 1, 2003; 49(8): 1245 - 1247.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
T. Nakanishi, K. Iguchi, and A. Shimizu
Method for Hemoglobin A1c Measurement Based on Peptide Analysis by Electrospray Ionization Mass Spectrometry with Deuterium-labeled Synthetic Peptides as Internal Standards
Clin. Chem., May 1, 2003; 49(5): 829 - 831.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
D. B. Sacks, D. E. Bruns, D. E. Goldstein, N. K. Maclaren, J. M. McDonald, and M. Parrott
Guidelines and Recommendations for Laboratory Analysis in the Diagnosis and Management of Diabetes Mellitus
Clin. Chem., March 1, 2002; 48(3): 436 - 472.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
W. L. Roberts, B. K. De, D. Brown, C. M. Hanbury, J. D. Hoyer, W. G. John, T. L. Lambert, R. B. Lundell, C. Rohlfing, and R. R. Little
Effects of Hemoglobin C and S Traits on Eight Glycohemoglobin Methods
Clin. Chem., February 1, 2002; 48(2): 383 - 385.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
L. Bry, P. C. Chen, and D. B. Sacks
Effects of Hemoglobin Variants and Chemically Modified Derivatives on Assays for Glycohemoglobin
Clin. Chem., February 1, 2001; 47(2): 153 - 163.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
T. Nakanishi, A. Miyazaki, K. Iguchi, and A. Shimizu
Effect of Hemoglobin Variants on Routine Glycohemoglobin Measurements Assessed by a Mass Spectrometric Method
Clin. Chem., October 1, 2000; 46(10): 1689 - 1692.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
E. L. Frank, L. Moulton, R. R. Little, H.-M. Wiedmeyer, C. Rohlfing, and W. L. Roberts
Effects of Hemoglobin C and S Traits on Seven Glycohemoglobin Methods
Clin. Chem., June 1, 2000; 46(6): 864 - 867.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
W. L. Roberts, J. M. Chiasera, and K. M. Ward-Cook
Glycohemoglobin Results in Samples with Hemoglobin C or S Trait: A Comparison of Four Test Systems
Clin. Chem., June 1, 1999; 45(6): 906 - 909.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
C. W. Weykamp, K. Miedema, T. de Haan, and C. J.A. Doelman
Carbamylated Hemoglobin Interference in Glycohemoglobin Assays
Clin. Chem., March 1, 1999; 45(3): 438 - 440.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
E. Bisse, N. Zorn, A. Eigel, M. Lizama, P. Huaman-Guillen, W. Marz, A. Van Dorsselaer, and H. Wieland
Hemoglobin Rambam (ß69[E13]Gly->Asp), a pitfall in the assessment of diabetic control: characterization by electrospray mass spectrometry and HPLC
Clin. Chem., October 1, 1998; 44(10): 2172 - 2177.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
C. J. A. Doelman, C. W. M. Siebelder, W. A. Nijhof, C. W. Weykamp, J. Janssens, and T. J. Penders
Capillary electrophoresis system for hemoglobin A1c determinations evaluated
Clin. Chem., April 1, 1997; 43(4): 644 - 648.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
G. Halwachs-Baumann, S. Katzensteiner, W. Schnedl, P. Purstner, T. Pieber, and M. Wilders-Truschnig
Comparative evaluation of three assay systems for automated determination of hemoglobin A1c
Clin. Chem., March 1, 1997; 43(3): 511 - 517.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
P. Holownia, E. Bishop, D. J. Newman, W. G. John, and C. P. Price
Adaptation of latex-enhanced assay for percent glycohemoglobin to a Dade Dimension(R) analyzer
Clin. Chem., January 1, 1997; 43(1): 76 - 84.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1993 by the American Association for Clinical Chemistry.