Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 39: 1825-1830, 1993;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gamache, P. H.
Right arrow Articles by Acworth, I. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gamache, P. H.
Right arrow Articles by Acworth, I. N.

Clinical Chemistry, Vol 39, 1825-1830, Copyright © 1993 by American Association for Clinical Chemistry

Urinary metanephrine and normetanephrine determined without extraction by using liquid chromatography and coulometric array detection

PH Gamache, ML Kingery and IN Acworth
ESA, Inc., Bedford, MA 01730.

We describe a procedure for the direct measurement of metanephrine (MN) and normetanephrine (NMN) in hydrolyzed urine, using HPLC with coulometric array detection. Acid-hydrolyzed samples were diluted and filtered before separation by isocratic reversed-phase ion-pair chromatography. Eight serial coulometric sensors, set at incrementally increasing anodic potentials, were used to screen lower-oxidizing interferences and provide stepwise oxidation of the metanephrines. Voltammetric behavior across three adjacent sensors was used to assess resolution and aid in peak identification. Values obtained in commercial controls were consistently within the specified target range. Variability, expressed as CV, was 5.45-9.22% between runs and 1.60-4.52% within-run for both compounds. The limit of detection was 2.6 micrograms/L for MN and 2.8 micrograms/L for NMN, with a linear response to 15.0 mg/L for both analytes. Results from patients' samples correlated well with those by a method involving dual ion-exchange extraction (r = 0.963, n = 82 for MN; r = 0.9768, n = 83 for NMN). This procedure provided high selectivity and objective peak purity information while greatly simplifying sample preparation.


The following articles in journals at HighWire Press have cited this article:


Home page
 J. Pharmacol. Exp. Ther.Home page
J. Riba, M. Valle, G. Urbano, M. Yritia, A. Morte, and M. J. Barbanoj
Human Pharmacology of Ayahuasca: Subjective and Cardiovascular Effects, Monoamine Metabolite Excretion, and Pharmacokinetics
J. Pharmacol. Exp. Ther., July 1, 2003; 306(1): 73 - 83.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
D. K. Crockett, E. L. Frank, and W. L. Roberts
Rapid Analysis of Metanephrine and Normetanephrine in Urine by Gas Chromatography-Mass Spectrometry
Clin. Chem., February 1, 2002; 48(2): 332 - 337.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
J. Wassell, P. Reed, J. Kane, and C. Weinkove
Freedom from Drug Interference in New Immunoassays for Urinary Catecholamines and Metanephrines
Clin. Chem., December 1, 1999; 45(12): 2216 - 2223.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1993 by the American Association for Clinical Chemistry.