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Clinical Chemistry 4: 43-48, 1958;
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Clinical Chemistry, Vol 4, 43-48, Copyright © 1958 by the American Association for Clinical Chemistry

Serum Copper and Iron on a Single Sample

B. Zak 1 and N. Ressler 2

1 Department of Pathology, Wayne State University College of Medicine and Detroit Receiving Hospital, Detroit, Mich.
2 Department of Medicine, Wayne County General Hospital, Eloise, Mich.

A procedure has been described for the quantitative determination of copper and iron in one serum sample. The sera are wet ashed to destroy organic material, leaving inorganic salts available in the residue for the subsequent spectrophotometric determination of iron as the ferrous-1,10-phenanthroline complex in the aqueous phase, and copper as the cuprous-2,9-dimethyl-4,7-diphenyl-1,10-phenanthroline in an organic extract phase.

Submitted on March 27, 1957




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