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Clinical Chemistry 4: 278-285, 1958;
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Clinical Chemistry, Vol 4, 278-285, Copyright © 1958 by the American Association for Clinical Chemistry

Practical Procedure for Estimation of Corticosterone or Hydrocortisone

Robert H. Silber 1, Robert D. Busch 1, and Raymond Oslapas 1

1 Merck Institute for Therapeutic Research, Rahway, N. J.

A simplified fluorometric procedure for corticosterone or hydrocortisone has been described. In spite of certain specificity limitations, it has practical applications for the estimation of these steroids in samples that are small in volume or low in concentration, or in studies that are designed primarily to demonstrate increases or decreases in adrenocortical hormones in plasma or adrenals without requiring absolute specificity. For rat, human, monkey, or guinea pig plasma, 0.5 ml. suffices for an analysis. One rat adrenal or a fraction of a guinea pig adrenal usually contains enough steroid for a single determination.

A procedure also has been described which can be applied to rat plasma to correct for interfering fluorescent material.

Submitted on May 7, 1958




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