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Clinical Chemistry 4: 304-310, 1958;
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Clinical Chemistry, Vol 4, 304-310, Copyright © 1958 by the American Association for Clinical Chemistry

Determination of Lipoproteins by Paper Electrophoresis

Pierre Moinat 1, Walter Appel 1, and Elizabeth F. Tuller 1

1 Baker Clinic Research Laboratory, New England Deaconess Hospital, and the Department of Nutrition, Harvard School of Public Health, Boston, Mass.

A reproducible technic is presented for the staining of lipoproteins on paper. This method gives a greater total uptake of dye than previously described procedures, especially by the alpha-lipoproteins. The staining is accomplished with a solution of Sudan Black B in a mixture of methyl and isopropyl alcohols and the excess dye is removed by wash solution of 30% isopropanol.

Submitted on February 28, 1958







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Copyright © 1958 by the American Association for Clinical Chemistry.