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Clinical Chemistry 4: 462-475, 1958;
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Clinical Chemistry, Vol 4, 462-475, Copyright © 1958 by the American Association for Clinical Chemistry

An Enzymatic Method for Glucose Determination in Body Fluids

Eliot F. Beach 1 and James J. Turner 1

1 Biochemical Laboratory, Metropolitan Life Insurance Company, New York 10, N. Y.

1. Details are given for the qualitative detection of glucose in urine and for its quantitative determination in urine and blood filtrates by an enzymatic procedure.

2. The method, which is highly specific, is based upon the release of hydrogen peroxide from glucose during its oxidation by glucose oxidase. The peroxide is detected by a suitable indicator in the presence of horseradish root peroxidase.

3. The results yielded by the new procedures have been compared with those obtained by other accepted methods.

Submitted on July 29, 1958






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Copyright © 1958 by the American Association for Clinical Chemistry.