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Clinical Chemistry 4: 484-495, 1958;
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Clinical Chemistry, Vol 4, 484-495, Copyright © 1958 by the American Association for Clinical Chemistry

A New Method for Starch Gel Electrophoresis of Human Hemoglobins, with Special Reference to the Determination of Hemoglobin A2

C. A. J. Goldberg 1

1 William Pepper Laboratory of Clinical Medicine, University of Pennsylvania, Philadelphia, Pa.

A method for starch gel electrophoresis of hemoglobins is presented in which a modified Lintner starch is used for the preparation of the gel. A discontinuous buffer system of tris-EDTA-borate/barbital is used as the electrolyte medium because of its superior resolving power.

Hemoglobin A2 values, obtained with this method, of healthy individuals, patients with thalassemia, and those with various anemias of nonthalassemic origin are presented.

Submitted on July 29, 1958




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Copyright © 1958 by the American Association for Clinical Chemistry.