Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 40: 116-123, 1994;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Brailly, H.
Right arrow Articles by vanSnick, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brailly, H.
Right arrow Articles by vanSnick, J.

Clinical Chemistry, Vol 40, 116-123, Copyright © 1994 by American Association for Clinical Chemistry

Total interleukin-6 in plasma measured by immunoassay

H Brailly, FA Montero-Julian, CE Zuber, S Flavetta, J Grassi, F Houssiau and J vanSnick
Immunotech S.A. 130, Marseille, France.

Determinations of total cytokine concentration in biological fluids by immunoassays face two major problems: the biochemical heterogeneity of the analyte and the interference of cytokine-binding proteins. We developed an ultrasensitive enzyme immunoassay for interleukin-6 (IL- 6), using monoclonal antibodies and acetylcholinesterase as the tracer enzyme. The antibodies recognized recombinant and glycosylated forms of IL-6 equally. The antibodies measured dimeric recombinant IL-6, yet we could not detect IL-6 oligomers in plasma samples. We investigated the potential interference of soluble IL-6 receptor (sIL-6R), which is present at high concentrations in plasma samples (1 to 2 nmol/L). Heat treatment of the sample obviated the sIL-6R interference. Using calibrators in a plasma matrix, we demonstrated by fractionation, dilution, and recovery experiments that the immunoassay accurately measured total IL-6 in both normal and pathological serum and plasma samples.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
L. M. Bathrellos, E. S. Lianidou, and P. C. Ioannou
A Highly Sensitive Enzyme-amplified Lanthanide Luminescence Immunoassay for Interleukin 6
Clin. Chem., June 1, 1998; 44(6): 1351 - 1353.
[Full Text] [PDF]

Home page
 CVIHome page
U. Sack, U. Burkhardt, M. Borte, H. Schädlich, K. Berg, and F. Emmrich
Age-Dependent Levels of Select Immunological Mediators in Sera of Healthy Children
Clin. Vaccine Immunol., January 1, 1998; 5(1): 28 - 32.
[Abstract] [Full Text]

Home page
 BloodHome page
M. Kalai, F. A. Montero-Julian, J. Grotzinger, V. Fontaine, P. Vandenbussche, R. Deschuyteneer, A. Wollmer, H. Brailly, and J. Content
Analysis of the Human Interleukin-6/Human Interleukin-6 Receptor Binding Interface at the Amino Acid Level: Proposed Mechanism of Interaction
Blood, February 15, 1997; 89(4): 1319 - 1333.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1994 by the American Association for Clinical Chemistry.