Clinical Chemistry, Vol 40, 30-37, Copyright © 1994 by American Association for Clinical Chemistry

Two-site enzyme immunoassay of CD4 and CD8 molecules on the surface of T lymphocytes from healthy subjects and HIV-1-infected patients

D Carriere, C Fontaine, AM Berthier, AM Rouquette, P Carayon, M Laprode, R Juillard, A Jansen, P Paoli and F Paolucci
Sanofi Recherche, Montpellier, France.

A highly sensitive two-site enzyme immunoassay (Capcellia) was developed to determine the concentration of CD4 and CD8 molecules expressed on the surface of human T lymphocytes. This assay, performed in one step (20 min), involves the specific immunocapture of T lymphocytes and reaction of the CD4 or CD8 molecules with an enzyme- labeled monoclonal antibody (mAb). The results were expressed as molar concentrations of the T-cell markers on the basis of results obtained with calibrated CD4 and CD8 standards. The assay was sensitive enough to detect 0.4 pmol/L CD4 or 0.8 pmol/L CD8, which corresponded to approximately 20 x 10(6) CD4+ or CD8+ T cells per liter of blood. Mean concentrations in healthy adults were 17.2 pmol/L for CD4 and 22.1 pmol/L for CD8. The CD4 concentration was < 8 pmol/L in 50% of HIV-1- infected patients and in 95% of AIDS patients. Given the epitopic specificity of the mAb to CD4 we used, these values correspond to the concentration of CD4 molecules free of envelope glycoprotein (gp)120.

The following articles in journals at HighWire Press have cited this article:

				D. Carriere, J. P. Vendrell, C. Fontaine, A. Jansen, J. Reynes, I. Pages, C. Holzmann, M. Laprade, and B. Pau Whole Blood Capcellia CD4/CD8 Immunoassay for Enumeration of CD4+ and CD8+ Peripheral T Lymphocytes Clin. Chem., January 1, 1999; 45(1): 92 - 97. [Abstract] [Full Text] [PDF]