Clinical Chemistry
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Clinical Chemistry 40: 71-73, 1994;
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Clinical Chemistry, Vol 40, 71-73, Copyright © 1994 by American Association for Clinical Chemistry

Measurement of blood volume with an enriched stable isotope of chromium (53Cr) and isotope dilution by combined gas chromatography-mass spectrometry

C Veillon, KY Patterson, DA Nagey and AM Tehan
Beltsville Human Nutrition Research Center, US Department of Agriculture, MD 20705.

We used an established isotope dilution gas chromatography-mass spectrometry method to measure blood volumes with an enriched stable isotope of chromium. The results were compared with those of the conventional method, which involves radioactive 51Cr. The two methods were compared simultaneously in two male subjects of different sizes, and essentially identical volumes were obtained. The isotope dilution method is insensitive to contamination with natural (unenriched) chromium and can be used to measure all of the stable isotopes of chromium. The method has potential applications in simultaneous erythrocyte survival studies. The absence of radioactivity makes possible an accurate means of studying blood volume changes in children and during pregnancy.


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Copyright © 1994 by the American Association for Clinical Chemistry.