Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 40: 1904-1908, 1994;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shihabi, Z. K.
Right arrow Articles by Oles, K. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shihabi, Z. K.
Right arrow Articles by Oles, K. S.

Clinical Chemistry, Vol 40, 1904-1908, Copyright © 1994 by American Association for Clinical Chemistry

Felbamate measured in serum by two methods: HPLC and capillary electrophoresis

ZK Shihabi and KS Oles
Department of Pathology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157.

We have developed two methods for determining serum concentrations of felbamate, a new anticonvulsant drug. The first method is based on protein precipitation with acetonitrile, followed by HPLC. The between- run CV for this method is 5.7% (mean 55 mg/L), and the linearity extends from 5 to 175 mg/L. Results by this method compared well with those by an HPLC method based on chloroform extraction (r = 0.98, n = 21). In the second method, based on micellar electrokinetic capillary chromatography, the drug is measured by capillary electrophoresis with direct injection of serum. This method can be completed in 5 min compared with 12 min for the HPLC method, and there is no need for sample extraction. The between-run CV is 5.2% (mean 58 mg/L) and the linearity range is 5-160 mg/L. Results of this direct method correlated well (r = 0.98, n = 37) with those by the HPLC assay. The mean trough serum concentration of felbamate in 123 patients taking this drug was 44.9 mg/L (range 12-129 mg/L).




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1994 by the American Association for Clinical Chemistry.