Clinical Chemistry
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Clinical Chemistry 40: 2254-2259, 1994;
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Clinical Chemistry, Vol 40, 2254-2259, Copyright © 1994 by American Association for Clinical Chemistry

Detection of autoantibodies to amylase by ELISA: comparison of detection of macroamylase and free autoantibody

GL Hortin, AL Summerfield, TR Wilhite, CH Smith, EL Branum, JF O'Brien and M Landt
Department of Pathology, University of Alabama at Birmingham 35233-7331.

New ELISAs for detecting macroamylase or free autoantibodies to amylase were tested with 48 samples that had been characterized by gel chromatography and electrophoresis. The macroamylase ELISA, with anti- IgG or anti-IgA for detection, detected macroamylase in 28 of 33 samples known to contain macroamylase (85% sensitivity), whereas the ELISA for free autoantibody to amylase was positive for only 11 samples. Specificities of both ELISAs were 93%. Among 28 true positives detected with the macroamylase ELISA, 22 contained IgA, 3 contained IgG, and 3 contained both immunoglobulins. Detection of IgM added no true positives. ELISA responses (y) were proportional to log [macroamylase concentration by chromatography (x)] from 0 to 1200 U/L: y = 5.15 x + 1.66; r = 0.72; Sy x = 1.65. As new tools for detecting macroenzymes consisting of enzyme-autoantibody complexes, the ELISAs show that some autoantibodies are detected more sensitively as antibody- antigen complexes than as free antibody.


The following articles in journals at HighWire Press have cited this article:


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G. Barera, E. Bazzigaluppi, M. Viscardi, F. Renzetti, C. Bianchi, G. Chiumello, and E. Bosi
Macroamylasemia Attributable to Gluten-Related Amylase Autoantibodies: A Case Report
Pediatrics, June 1, 2001; 107 (6): e93 - e93.
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