Clinical Chemistry
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Clinical Chemistry 40: 190-196, 1994;
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Clinical Chemistry, Vol 40, 190-196, Copyright © 1994 by American Association for Clinical Chemistry

Electrophoretic fractionation of 5'-nucleotidase

M Panteghini
Laboratorio Analisi Chimico-Cliniche, Spedali Civili, Brescia, Italy.

Human isonucleotidases were separated by electrophoresis on a cellulose acetate membrane. Three 5'-nucleotidase forms, NTP1, NTP2, and NTP3, were resolved with this method and quantified by densitometry. The procedure was not only simple and rapid but also sufficiently precise (between-run CV < 20%) and sensitive (detected nucleotidase fractions of > 0.5 U/L). The effects of various treatments (heat, neuraminidase, glycosidases, proteases, lectins, and detergents) on the electrophoretic pattern of 5'-nucleotidase were studied. NTP1 (mean 12% of total 5'-nucleotidase, SD 5%), NTP2 (mean 30%, SD 8%), and NTP3 (mean 58%, SD 8%) were found in all normal persons studied. The increase in total 5'-nucleotidase in patients with hepatobiliary disease was mainly due to the NTP1 isoform.


The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
F. Pagani and M. Panteghini
5'-Nucleotidase in the Detection of Increased Activity of the Liver Form of Alkaline Phosphatase in Serum
Clin. Chem., November 1, 2001; 47(11): 2046 - 2048.
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Copyright © 1994 by the American Association for Clinical Chemistry.