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Clinical Chemistry, Vol 40, 347-357, Copyright © 1994 by American Association for Clinical Chemistry
LJ Kricka
Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia 19104.
Selected recent advances in immunoassay are reviewed. Development has continued on new labels (beta-lactamase, pyrophosphatase, luciferases, photoproteins, pyridopyridazines, europium cryptates, metal carbonyl complexes, porphines, phosphors) and label-detection methods (e.g., chemiluminescence assays, thermometric assays, NADP(+)- and FADP-based coupled assays). Various methods have been explored to increase assay sensitivity, including label amplification via catalyzed reporter deposition (peroxidase label) and immuno-polymerase chain reaction (DNA label). The focus of new immunoassay strategies has been on improved reliability (bispecific antibodies), assay simplification (piezoelectric and surface plasmon resonance immunosensors, phase- modulation fluorescence spectroscopy, polymerized bilayer assemblies), and simultaneous multianalyte testing (e.g., quadruple labeling with lanthanides, one-step test devices for drugs of abuse).
The following articles in journals at HighWire Press have cited this article:
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  X. L. Tang, M. S. Peppler, R. T. Irvin, and M. R. Suresh Use of Bispecific Antibodies in Molecular Velcro Assays Whose Specificity Approaches the Theoretical Limit of Immunodetection for Bordetella pertussis Clin. Vaccine Immunol., July 1, 2004; 11(4): 752 - 757. [Abstract] [Full Text] [PDF]
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 T. Soukka, J. Paukkunen, H. Harma, S. Lonnberg, H. Lindroos, and T. Lovgren Supersensitive Time-resolved Immunofluorometric Assay of Free Prostate-specific Antigen with Nanoparticle Label Technology Clin. Chem., July 1, 2001; 47(7): 1269 - 1278. [Abstract] [Full Text] [PDF]
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W. Qian, D. Yao, F. Yu, B. Xu, R. Zhou, X. Bao, and Z. Lu Immobilization of Antibodies on Ultraflat Polystyrene Surfaces Clin. Chem., September 1, 2000; 46(9): 1456 - 1463. [Abstract] [Full Text] [PDF]
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L. J. Kricka Nucleic Acid Detection Technologies — Labels, Strategies, and Formats Clin. Chem., April 1, 1999; 45(4): 453 - 458. [Abstract] [Full Text] [PDF]
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C. P. Price The Evolution of Immunoassay as Seen Through the Journal Clinical Chemistry Clin. Chem., October 1, 1998; 44(10): 2071 - 2074. [Full Text] [PDF]
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T. Lovgren, P. Heinonen, P. Lehtinen, H. Hakala, J. Heinola, R. Harju, H. Takalo, V.-M. Mukkala, R. Schmid, H. Lonnberg, et al. Sensitive bioaffinity assays with individual microparticles and time-resolved fluorometry Clin. Chem., October 1, 1997; 43(10): 1937 - 1943. [Abstract] [Full Text] [PDF]
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L. J. Kricka Optical Tweezers and Immunoassay Clin. Chem., February 1, 1997; 43(2): 251 - 253. [Full Text] [PDF]
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G. S. Makowski and S. M. Hopfer Measurement of Maternal Folate Status and Risk of Neural Tube Defects Clin. Chem., January 1, 1997; 43(1): 185 - 186. [Full Text]
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