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Clinical Chemistry 40: 411-416, 1994;
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Clinical Chemistry, Vol 40, 411-416, Copyright © 1994 by American Association for Clinical Chemistry

Retinol, alpha-tocopherol, lutein/zeaxanthin, beta-cryptoxanthin, lycopene, alpha-carotene, trans-beta-carotene, and four retinyl esters in serum determined simultaneously by reversed-phase HPLC with multiwavelength detection

AL Sowell, DL Huff, PR Yeager, SP Caudill and EW Gunter
Division of Environmental Health Laboratory Sciences, Centers for Disease Control and Prevention, Public Health Service, Atlanta, GA 30341-3724.

We describe the use of HPLC with multiwavelength detection to measure retinol, alpha-tocopherol, lutein/zeaxanthin, beta-cryptoxanthin, lycopene, alpha-carotene, trans-beta-carotene, beta-carotene, and the linoleate, oleate, palmitate, and stearate esters of retinol in a single 200-microL serum sample. The method is sensitive enough to detect individual retinyl esters in fasting serum from a nonhyperlipidemic population and requires only 12 min for each sample. Serum concentration ranges and means are reported for retinol, alpha- tocopherol, lutein/zeaxanthin, beta-cryptoxanthin, lycopene, alpha- carotene, trans-beta-carotene, and the sum of the retinyl esters from serum analyses of 3480 participants from several different studies.


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