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Clinical Chemistry 40: 625-629, 1994;
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Clinical Chemistry, Vol 40, 625-629, Copyright © 1994 by American Association for Clinical Chemistry

Automated latex agglutination immunoassay of serum ferritin with a centrifugal analyzer

JM Simo, J Joven, X Cliville and T Sans
Centre de Recerca Biomedica, Hospital de Sant Joan de Reus, Spain.

A considerable demand for convenient, rapid, inexpensive assays of ferritin in serum has been generated in recent years in hospital laboratories and blood banks. We describe a simple and rapid particle- enhanced turbidimetric immunoassay suitable for routine application in a Monarch 2000 centrifugal analyzer with commercially available reagents. This fully automated assay (y) requires no pretreatment of sample, and correlation with a two-step sandwich ELISA (x) is excellent (y = 1.018x + 0.397, Sy/x = 0.027). The analytical range extends from 5 to 900 micrograms/L. Intraassay imprecision (CV) ranged from 1.1% to 5% for various specimen concentrations. Interassay imprecision ranged from 2.2% for above-normal concentrations (755 micrograms/L) to 9.5% for low concentrations (39 micrograms/L). No specimen-related carryover was detected. The method has been useful in our predeposit autologous blood transfusion program for rapid assessment of iron status in patients undergoing repeated phlebotomies.


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Copyright © 1994 by the American Association for Clinical Chemistry.