Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 40: 630-636, 1994;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sestini, R.
Right arrow Articles by Pazzagli, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sestini, R.
Right arrow Articles by Pazzagli, M.

Clinical Chemistry, Vol 40, 630-636, Copyright © 1994 by American Association for Clinical Chemistry

Measuring c-erbB-2 oncogene amplification in fresh and paraffin- embedded tumors by competitive polymerase chain reaction

R Sestini, C Orlando, L Zentilin, S Gelmini, P Pinzani, S Bianchi, C Selli, M Giacca and M Pazzagli
Department of Clinical Physiopathology, University of Florence, Italy.

We present an original application of competitive polymerase chain reaction (PCR) for measuring oncogene amplification in DNA from human tumors by simultaneous PCR amplification of genomic DNA with fixed amounts of an internal standard (competitor DNA). Competitors share the same sequence as the target genes but contain an additional 15- to 20- base-pair insert, which allows resolution of the amplified products after polyacrylamide gel electrophoresis and ethidium bromide staining. The gene copy number is derived from the ratio between the intensities of the bands corresponding to the amplified products. Using this procedure, we measured c-erbB-2 amplification in breast and bladder carcinomas in both fresh tumor tissues and paraffin-embedded tissue samples and assessed the precision, sensitivity, and accuracy of the assay. Competitive PCR is a simple, reliable, and accurate method for the evaluation of c-erbB-2 amplification and is potentially suitable for use in the clinical laboratory.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
C. C. Raggi, M. L. Bagnoni, G. P. Tonini, M. Maggi, G. Vona, P. Pinzani, K. Mazzocco, B. De Bernardi, M. Pazzagli, and C. Orlando
Real-Time Quantitative PCR for the Measurement of MYCN Amplification in Human Neuroblastoma with the TaqMan Detection System
Clin. Chem., November 1, 1999; 45(11): 1918 - 1924.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
F. Revillion, L. Hornez, and J.-P. Peyrat
Quantification of c-erbB-2 gene expression in breast cancer by competitive RT-PCR
Clin. Chem., November 1, 1997; 43(11): 2114 - 2120.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
S. Gelmini, C. Orlando, R. Sestini, G. Vona, P. Pinzani, L. Ruocco, and M. Pazzagli
Quantitative polymerase chain reaction-based homogeneous assay with fluorogenic probes to measure c-erbB-2 oncogene amplification
Clin. Chem., May 1, 1997; 43(5): 752 - 758.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1994 by the American Association for Clinical Chemistry.