Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 40: 705-709, 1994;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Aoki, Y.
Right arrow Articles by Rustgi, A. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Aoki, Y.
Right arrow Articles by Rustgi, A. K.

Clinical Chemistry, Vol 40, 705-709, Copyright © 1994 by American Association for Clinical Chemistry

Radiolabeled polymerase chain reaction assay for detection of ras oncogene point mutations in tumors

Y Aoki, JC Lee, S Pillai, KJ Isselbacher and AK Rustgi
Massachusetts General Hospital Cancer Center, Harvard Medical School, Charlestown 02129.

The human ras gene plays a fundamental role in the transduction of extracellular signals to the nucleus, thereby regulating cell growth and differentiation. Point mutations in the ras gene convert it into a transforming oncogene that has been found in many solid and hematologic malignancies. We describe a rapid and sensitive assay based on a radiolabeled polymerase chain reaction followed by restriction enzyme digestion that we have adapted for differentiating between the wild- type and mutant ras genes. This assay should prove useful in the analysis of ras gene point mutations in clinical tumor specimens in which ras oncogene activation is an early event in carcinogenesis.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
E. Lopez-Crapez, C. Chypre, J. Saavedra, J. Marchand, and J. Grenier
Rapid and large-scale method to detect K-ras gene mutations in tumor samples
Clin. Chem., June 1, 1997; 43(6): 936 - 942.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1994 by the American Association for Clinical Chemistry.