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Clinical Chemistry 40: 749-753, 1994;
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Clinical Chemistry, Vol 40, 749-753, Copyright © 1994 by American Association for Clinical Chemistry

Time-resolved immunofluorometric assay and specimen storage conditions for measuring urinary gonadotropins

M Saketos, N Sharma, T Adel, M Raghuwanshi and N Santoro
Mount Sinai School of Medicine, Department of Obstetrics, Gynecology and Reproductive Science, New York, NY 10029.

We optimized storage conditions and validated a sensitive immunofluorometric assay (IFMA) for urinary gonadotropins. Assay linearity and parallelism for luteinizing hormone (LH) and follicle- stimulating hormone (FSH) was observed to 0.04 IU/L. Urinary LH and FSH were unaffected by changes of osmolarity from 0.5 to 3.0 mOsm/kg, and from pH 4.5 to 10.5. Serum and urine measurements of the hormones correlated well over a wide range of values: for LH, R2 = 0.94, P < 0.01; for FSH, R2 = 0.71, P < 0.01 (n = 304). Preservation of urine with glycerol (70 mL/L) and storage at -20 degrees C yielded > 80% recovery of LH and FSH after 51 weeks; this was comparable with recovery for acetone extracts of urine. Untreated urine showed loss of activity by 4 weeks of storage. Preserving urine specimens with glycerol is a simple method of storage for longitudinal study and compares favorably with acetone extraction. IFMAs can measure urinary gonadotropins reproducibly over a wide range of pH and osmotic conditions.


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Copyright © 1994 by the American Association for Clinical Chemistry.