Clinical Chemistry
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Clinical Chemistry 40: 754-757, 1994;
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Clinical Chemistry, Vol 40, 754-757, Copyright © 1994 by American Association for Clinical Chemistry

Immunoradiometric assay of human intact proinsulin applied to patients with type 2 diabetes, impaired glucose tolerance, and hyperandrogenism

D Chevenne, J Ruiz, L Lohmann, A Laudat, H Leblanc, IP Gray, P Passa and D Porquet
Hopital Robert Debre, Laboratoire de Biochimie-Hormonologie, Paris, France.

We describe an immunoradiometric assay for human intact proinsulin in serum. In this method, one monoclonal antibody, coated onto polyacrylamide beads, cross-reacts with proinsulins and insulin. A sandwich is formed with intact proinsulin, split (65-66) proinsulin, and des (64-65) proinsulin binding with an 125I-labeled monoclonal antibody specific for an epitope at the intact B-C junction of proinsulin. Because split (65-66) and des (64-65) proinsulin concentrations are very low in serum, this assay essentially measures intact proinsulin. When we used 1-mL serum samples, the mean detection limit was 0.4 pmol/L. Mean proinsulin concentrations (pmol/L) were 3.4 (range 1-9.1) in healthy fasting subjects, 28.5 (9.7-101) in patients with type 2 diabetes (treated with metformin and sulfonylureas), 5.0 (1.6-9.3) in women with hyperandrogenism and normal insulinemia, 10.3 (2.6-36) in women with hyperandrogenism and hyperinsulinemia, and 8.5 (4.8-21.3) in patients with impaired glucose tolerance.


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 Clin. Chem.Home page
P. Houssa, B. Dinesen, M. Deberg, B. H. Frank, C. Van Schravendijk, F. Sodoyez-Goffaux, and J.-C. Sodoyez
First direct assay for intact human proinsulin
Clin. Chem., July 1, 1998; 44(7): 1514 - 1519.
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Copyright © 1994 by the American Association for Clinical Chemistry.