Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 40: 1485-1488, 1994;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mendis, S.
Right arrow Articles by Euler, D. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mendis, S.
Right arrow Articles by Euler, D. E.

Clinical Chemistry, Vol 40, 1485-1488, Copyright © 1994 by American Association for Clinical Chemistry

Pentane and isoprene in expired air from humans: gas-chromatographic analysis of single breath

S Mendis, PA Sobotka and DE Euler
Department of Medicine/Cardiology, Loyola University Medical Center, Maywood, IL 60153.

Both pentane and isoprene are excreted in human breath. Although pentane is considered an index of lipid peroxidation, the significance of isoprene is unknown. Having a similar boiling point, these two hydrocarbons are difficult to separate by gas chromatography. We separated pentane from isoprene on both a Poraplot Q and a Poraplot U column, injecting single-breath samples directly into a gas chromatograph. The breath samples were pressurized to 800 mmHg to increase the amount of sample volume delivered to the column. In a group of 43 healthy volunteers, the concentrations of end-expiratory pentane and isoprene were 0.57 +/- 0.3 and 7.05 +/- 3.53 nmol/L, respectively. There was a significant linear correlation (r = 0.57, P < 0.0001) between age and pentane concentration in expired air; isoprene showed no correlation with age or pentane concentrations. The age- related increase in pentane production suggests that oxidative stress may play a role in the aging process in humans. The method described should allow for rapid, inexpensive, serial measurement of expired pentane and isoprene.


The following articles in journals at HighWire Press have cited this article:


Home page
 J. Appl. Physiol.Home page
T. Karl, P. Prazeller, D. Mayr, A. Jordan, J. Rieder, R. Fall, and W. Lindinger
Human breath isoprene and its relation to blood cholesterol levels: new measurements and modeling
J Appl Physiol, August 1, 2001; 91(2): 762 - 770.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
W. Miekisch, J. K. Schubert, D. A. Vagts, and K. Geiger
Analysis of Volatile Disease Markers in Blood
Clin. Chem., June 1, 2001; 47(6): 1053 - 1060.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
S. Davies, P. Spanel, and D. Smith
A new 'online' method to measure increased exhaled isoprene in end-stage renal failure
Nephrol. Dial. Transplant., April 1, 2001; 16(4): 836 - 839.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. Smith, P. Spanel, and S. Davies
Trace gases in breath of healthy volunteers when fasting and after a protein-calorie meal: a preliminary study
J Appl Physiol, November 1, 1999; 87(5): 1584 - 1588.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
K. B. BECKMAN and B. N. AMES
The Free Radical Theory of Aging Matures
Physiol Rev, April 1, 1998; 78(2): 547 - 581.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1994 by the American Association for Clinical Chemistry.