Clinical Chemistry, Vol 40, 1559-1566, Copyright © 1994 by American Association for Clinical Chemistry

Plasma lipoprotein profiles of normocholesterolemic and hypercholesterolemic homozygotes for apolipoprotein E2(Arg158-->Cys) compared

SP Zhao, AH Smelt, AM Van den Maagdenberg, A Van Tol, TF Vroom, JA Gevers Leuven, A Van der Laarse and FM Van 't Hooft
Department of Cardiology, Medical Faculty, University of Leiden, The Netherlands.

We compared plasma lipoprotein profiles of 15 individuals with normocholesterolemic (plasma cholesterol 4.81 +/- 0.90 mmol/L) familial dysbetalipoproteinemia (NFD) and 15 patients with hypercholesterolemic (plasma cholesterol 10.61 +/- 2.32 mmol/L) familial dysbetalipoproteinemia (HFD), matched for age and sex. All subjects were homozygous for apoE2(Arg158-->Cys). Compared with 15 normolipidemic controls (plasma cholesterol 5.47 +/- 0.92 mmol/L), subjects with NFD and HFD had greater cholesterol concentrations of large very-low-density lipoprotein (VLDL1), small VLDL (VLDL2), and intermediate-density lipoprotein, each of which was correlated to their plasma total cholesterol concentration. VLDL1 and VLDL2 subfractions were enriched in cholesteryl ester, and plasma cholesteryl ester transfer protein activities were increased in both NFD and HFD; however, absolute changes were larger in HFD than in NFD. Concentrations of low-density lipoprotein cholesterol were lower in HFD (1.89 +/- 0.48 mmol/L) and NFD (1.56 +/- 0.36 mmol/L) than in normolipidemic controls (3.35 +/- 0.73 mmol/L). We conclude that all subjects homozygous for apoE2(Arg158-->Cys) show features of dysbetalipoproteinemia.

The following articles in journals at HighWire Press have cited this article:

				Y. Huang, S. W. Schwendner, S. C. Rall Jr., and R. W. Mahley Hypolipidemic and Hyperlipidemic Phenotypes in Transgenic Mice Expressing Human Apolipoprotein E2 J. Biol. Chem., November 15, 1996; 271(46): 29146 - 29151. [Abstract] [Full Text] [PDF]