Clinical Chemistry
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Clinical Chemistry 40: 1571-1574, 1994;
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Clinical Chemistry, Vol 40, 1571-1574, Copyright © 1994 by American Association for Clinical Chemistry

HPLC determination of polyethylene glycol 400 in urine: oligomeric profile in healthy and celiac disease subjects

A Oliva, H Armas and JB Farina
Departamento de Ingenieria Quimica y Tecnologia Farmaceutica, Facultad de Farmacia, Universidad de La Laguna, Tenerife, Spain.

The absorption of orally administered polyethylene glycol (PEG) has been used to assess intestinal permeability. We describe a simple HPLC technique to determine the oligomeric profile of PEG excreted in urine. We measured the total (%) PEG excreted in 6 h and the ratio of the four smallest oligomers to the three largest oligomers (expressed as mean percentages). The proposed method differentiates distinct groups of subjects with varying degrees of intestinal permeability detected by intestinal biopsy. The percent of PEG excreted and the oligomer ratio values for healthy subjects were, respectively, 30.1 +/- 3.87 and 0.35 +/- 0.03; for celiac patients on a gluten-free diet, 24.5 +/- 6.65 and 0.45 +/- 0.16; and for celiac patients, 15.0 +/- 5.93 and 1.12 +/- 0.55.




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Copyright © 1994 by the American Association for Clinical Chemistry.