Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 40: 1717-1721, 1994;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schwarz, S.
Right arrow Articles by Steinmetz, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schwarz, S.
Right arrow Articles by Steinmetz, A.

Clinical Chemistry, Vol 40, 1717-1721, Copyright © 1994 by American Association for Clinical Chemistry

Quantification of apolipoprotein A-IV in human plasma by immunonephelometry

S Schwarz, B Haas, C Luley, JR Schafer and A Steinmetz
Zentrum Innere Medizin, Abteilung Endokrinologie und Stoffwechsel, University of Marburg, Germany.

We developed a nephelometric procedure for determining concentrations of human plasma apolipoprotein (apo) A-IV. Results obtained correlate well with those measured by an established electroimmunodiffusion assay (r = 0.98; n = 56). Intra- and interassay CVs were 2.4% and 2.0%, respectively, indicating excellent precision and reproducibility. Various conditions of sample treatment, such as adequate storage, freezing, and thawing, did not affect results significantly. However, keeping samples at room temperature for 4 days led to a slight increase in measured values. Preincubation with a cholesterinesterase-detergent mixture abolished interference from triglyceride-rich lipoproteins, allowing assay of samples containing triglycerides as great as 10 g/L. The assay is easily applicable to clinical laboratories for routine diagnostic use, as shown with hypertriglyceridemic plasmas and samples with a broad range of apo A-IV concentrations.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
M. Rafat, A. M. Sattler, R. Hackler, M. Soufi, A. Steinmetz, B. Maisch, and J. R. Schaefer
Apolipoprotein A-IV in the Fed and Fasting States
Clin. Chem., July 1, 2004; 50(7): 1270 - 1271.
[Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
M. Orth, W. Weng, H. Funke, A. Steinmetz, G. Assmann, M. Nauck, J. Dierkes, A. Ambrosch, K. H. Weisgraber, R. W. Mahley, et al.
Effects of a Frequent Apolipoprotein E Isoform, ApoE4Freiburg (Leu28->Pro), on Lipoproteins and the Prevalence of Coronary Artery Disease in Whites
Arterioscler Thromb Vasc Biol, May 1, 1999; 19(5): 1306 - 1315.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1994 by the American Association for Clinical Chemistry.