Clinical Chemistry, Vol 40, 1800-1804, Copyright © 1994 by American Association for Clinical Chemistry

Microfabrication in silicon microphysiometry

GT Baxter, LJ Bousse, TD Dawes, JM Libby, DN Modlin, JC Owicki and JW Parce
Molecular Devices Corp., Sunnyvale, CA 94089.

Over the past 5 years, microphysiometry has proved an effective means for detecting physiological changes in cultured cells, particularly as a functional assay for the activation of many cellular receptors. To demonstrate the clinical relevance of this method, we have used it to detect bacterial antibiotic sensitivity and to discriminate between bacteriostatic and bacteriocidal concentrations. The light-addressable potentiometric sensor, upon which microphysiometry is based, is well suited for structural manipulations based on photolithography and micromachining, and we have begun to take advantage of this capability. We present results from a research instrument with eight separate assay channels on a 5-cm2 chip. We discuss the planned evolution of the technology toward high-through-put instruments and instruments capable of performing single-cell measurements.

The following articles in journals at HighWire Press have cited this article:

				T. J. Zieziulewicz, D. W. Unfricht, N. Hadjout, M. A. Lynes, and D. A. Lawrence Shrinking the Biologic World--Nanobiotechnologies for Toxicology Toxicol. Sci., August 1, 2003; 74(2): 235 - 244. [Abstract] [Full Text] [PDF]

				J. M. Libby Postantibiotic Effect in Escherichia coli Determined with Real-Time Metabolic Monitoring Antimicrob. Agents Chemother., January 1, 1998; 42(1): 78 - 82. [Abstract] [Full Text]