| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Clinical Chemistry, Vol 41, 1560-1566, Copyright © 1995 by American Association for Clinical Chemistry
B Gomez Jr, S Ardakani, J Ju, D Jenkins, MJ Cerelli, GY Daniloff and VT Kung
Metra Biosystems, Mountain View, CA 94043-3911, USA.
Alkaline phosphatase (ALP) is present in human serum in the form of several isoenzymes. The two major circulating ALP isoenzymes, bone and liver, are difficult to distinguish because they are the products of a single gene and differ only by posttranslational glycosylation. Quantitative measurement of bone ALP (BAP) activity in serum can provide an index for the rate of bone formation. Furthermore, increased BAP activity in serum is indicative of bone disorders. We describe a method in which serum samples are added to a microtiter plate coated with monoclonal anti-BAP antibody and incubated 3 h at room temperature. After the unbound materials are washed off, the bound BAP activity is measured by adding p-nitrophenyl phosphate substrate. The assay demonstrated no cross-reactivity to intestinal or placental ALP and only 3-8% cross-reactivity to liver ALP. The intraassay (n = 21) CVs were 3.9-5.9%, and interassay (n = 8) CVs were 4.4-7.0%. Comparisons of the assay (y) with an IRMA (x) and a wheat germ agglutinin precipitation method (x') gave regression equations of y = 1.32x-6.4, r = 0.99, and y = 1.41x' + 4.8, r = 0.99. The assay detected increased BAP in sera from patients with osteoporosis, Paget disease, osteomalacia, or primary hyperparathyroidism.
The following articles in journals at HighWire Press have cited this article:
|
|
 |
|
  L. Cleemann, B. E Hjerrild, A. L Lauridsen, L. Heickendorff, J. S Christiansen, L. Mosekilde, and C. H Gravholt Long-term hormone replacement therapy preserves bone mineral density in Turner syndrome Eur. J. Endocrinol., August 1, 2009; 161(2): 251 - 257. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R Hunt, J. M Beiseigel, and L. K Johnson Adaptation in human zinc absorption as influenced by dietary zinc and bioavailability Am. J. Clinical Nutrition, May 1, 2008; 87(5): 1336 - 1345. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Garnero, P. Vergnaud, and N. Hoyle Evaluation of a Fully Automated Serum Assay for Total N-Terminal Propeptide of Type I Collagen in Postmenopausal Osteoporosis Clin. Chem., January 1, 2008; 54(1): 188 - 196. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Johansen, K. Brasso, P. Iversen, B. Teisner, P. Garnero, P. A. Price, and I. J. Christensen Changes of Biochemical Markers of Bone Turnover and YKL-40 Following Hormonal Treatment for Metastatic Prostate Cancer Are Related to Survival Clin. Cancer Res., June 1, 2007; 13(11): 3244 - 3249. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Maeno, M. Inaba, S. Okuno, T. Yamakawa, E. Ishimura, and Y. Nishizawa Serum Concentrations of Cross-Linked N-Telopeptides of Type I Collagen: New Marker for Bone Resorption in Hemodialysis Patients Clin. Chem., December 1, 2005; 51(12): 2312 - 2317. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Atkinson, J. E Compston, N. E Day, M. Dowsett, and S. A Bingham The effects of phytoestrogen isoflavones on bone density in women: a double-blind, randomized, placebo-controlled trial Am. J. Clinical Nutrition, February 1, 2004; 79(2): 326 - 333. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Szulc, B. Claustrat, F. Marchand, and P. D. Delmas Increased Risk of Falls and Increased Bone Resorption in Elderly Men with Partial Androgen Deficiency: The MINOS Study J. Clin. Endocrinol. Metab., November 1, 2003; 88(11): 5240 - 5247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Suuriniemi, A. Mahonen, V. Kovanen, M. Alen, and S. Cheng Relation of PvuII site polymorphism in the COL1A2 gene to the risk of fractures in prepubertal Finnish girls Physiol Genomics, August 15, 2003; 14(3): 217 - 224. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Shioi, M. Katagi, Y. Okuno, K. Mori, S. Jono, H. Koyama, and Y. Nishizawa Induction of Bone-Type Alkaline Phosphatase in Human Vascular Smooth Muscle Cells: Roles of Tumor Necrosis Factor-{alpha} and Oncostatin M Derived From Macrophages Circ. Res., July 12, 2002; 91(1): 9 - 16. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Szulc, P. Garnero, B. Claustrat, F. Marchand, F. Duboeuf, and P. D. Delmas Increased Bone Resorption in Moderate Smokers with Low Body Weight: The Minos Study J. Clin. Endocrinol. Metab., February 1, 2002; 87(2): 666 - 674. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. R. Ebeling, J. D. Wark, S. Yeung, C. Poon, N. Salehi, G. C. Nicholson, and M. A. Kotowicz Effects of Calcitriol or Calcium on Bone Mineral Density, Bone Turnover, and Fractures in Men with Primary Osteoporosis: A Two-Year Randomized, Double Blind, Double Placebo Study J. Clin. Endocrinol. Metab., September 1, 2001; 86(9): 4098 - 4103. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. W. Woitge, E. Horn, A. V. Keck, B. Auler, M. J. Seibel, and M. Pecherstorfer Biochemical Markers of Bone Formation in Patients with Plasma Cell Dyscrasias and Benign Osteoporosis Clin. Chem., April 1, 2001; 47(4): 686 - 693. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Szulc, F. Munoz, B. Claustrat, P. Garnero, F. Marchand, F. Duboeuf, and P. D. Delmas Bioavailable Estradiol May Be an Important Determinant of Osteoporosis in Men: The MINOS Study J. Clin. Endocrinol. Metab., January 1, 2001; 86(1): 192 - 199. [Abstract] [Full Text]
|
|
|
|
|
|
D. R. Dufour, J. A. Lott, F. S. Nolte, D. R. Gretch, R. S. Koff, and L. B. Seeff Diagnosis and Monitoring of Hepatic Injury. I. Performance Characteristics of Laboratory Tests Clin. Chem., December 1, 2000; 46(12): 2027 - 2049. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kumeda, M. Inaba, H. Tahara, Y. Kurioka, T. Ishikawa, H. Morii, and Y. Nishizawa Persistent Increase in Bone Turnover in Graves' Patients with Subclinical Hyperthyroidism J. Clin. Endocrinol. Metab., November 1, 2000; 85(11): 4157 - 4161. [Abstract] [Full Text]
|
|
|
|
|
|
H. W. Woitge, H. Oberwittler, S. Heichel, A. Grauer, R. Ziegler, and M. J. Seibel Short- and Long-Term Effects of Ibandronate Treatment on Bone Turnover in Paget Disease of Bone Clin. Chem., May 1, 2000; 46(5): 684 - 690. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. B. Watts Clinical Utility of Biochemical Markers of Bone Remodeling Clin. Chem., August 1, 1999; 45(8): 1359 - 1368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-S. Tsai, M.-H. Jang, S. H.-J. Hsu, W.-C. Cheng, and M.-H. Chang Bone Alkaline Phosphatase Isoenzyme and Carboxy-Terminal Propeptide of Type-I Procollagen in Healthy Chinese Girls and Boys Clin. Chem., January 1, 1999; 45(1): 136 - 138. [Full Text] [PDF]
|
|
|
|
|
|
D. L. Broyles, R. G. Nielsen, E. M. Bussett, W. D. Lu, I. A. Mizrahi, P. A. Nunnelly, T. A. Ngo, J. Noell, R. H. Christenson, and B. C. Kress Analytical and clinical performance characteristics of Tandem-MP Ostase, a new immunoassay for serum bone alkaline phosphatase Clin. Chem., October 1, 1998; 44(10): 2139 - 2147. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. P. Price, T. P. Milligan, and C. Darte Direct comparison of performance characteristics of two immunoassays for bone isoform of alkaline phosphatase in serum Clin. Chem., November 1, 1997; 43(11): 2052 - 2057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H.-J. Hsu and K.-S. Tsai Different Age-Related Trends of Bone and Nonbone Forms of Alkaline Phosphatase in Chinese Men and Women Clin. Chem., January 1, 1997; 43(1): 186 - 188. [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
