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Clinical Chemistry 41: 1581-1584, 1995;
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Clinical Chemistry, Vol 41, 1581-1584, Copyright © 1995 by American Association for Clinical Chemistry

Reliability of salivary testosterone measurements: a multicenter evaluation

JM Dabbs Jr, BC Campbell, BA Gladue, AR Midgley, MA Navarro, GF Read, EJ Susman, LM Swinkels and CM Worthman
Department of Psychology, Georgia State University, Atlanta, USA.

The reliability of salivary testosterone assays was evaluated by nine laboratories in four countries. Each laboratory used its own RIA procedures to assay samples from a set of 100 male and 100 female subjects. Agreement among the laboratories on mean scores was within the range reported by Read (Ann N Y Acad Sci 1993; 694: 161-76). Overall agreement on individual scores, as indicated by the intraclass correlation coefficient computed within subjects across laboratories, was r = 0.87 for men and r = 0.78 for women. Mean agreement between each laboratory and the combined set of all other laboratories (via Fisher's Z-transformation) was r = 0.61 for men and r = 0.58 for women. We take these latter values to be the best estimates of the average reliability of laboratories in their ordering of individual samples.


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