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Clinical Chemistry, Vol 41, 1761-1767, Copyright © 1995 by American Association for Clinical Chemistry
M Nauck, K Winkler, W Marz and H Wieland
Department of Medicine, University Hospital of Freiburg, Germany.
Quantification of lipoprotein cholesterol was performed by enzymatic staining of cholesterol in a new agarose gel electrophoresis method that allows the separation of LDL, VLDL, HDL, and lipoprotein(a) [Lp(a)]. Lp(a) shows an electrophoretic mobility clearly distinct from VLDL and HDL. The total CVs of lipoprotein cholesterol varied between 2.7% and 3.9% for LDL, 7.8% and 23.2% for VLDL, 5.2% and 9.5% for HDL, and 6.8% and 16.4% for Lp(a). Comparison of LDL-, VLDL-, and HDL- cholesterol concentrations with the results of a combined ultracentrifugation and precipitation technique gave correlation coefficients of 0.961, 0.947, and 0.918, respectively; comparison of Lp(a)-cholesterol values with those of a nephelometric Lp(a) assay gave r = 0.906. The new electrophoretic assay has several advantages: It allows the quantification of Lp(a)-cholesterol; VLDL-cholesterol is not affected by Lp(a)-cholesterol; and the LDL-cholesterol fraction does not contain Lp(a)-cholesterol, as happens with LDL-cholesterol determined by ultracentrifugation and precipitation.
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F. Paultre, C. H. Tuck, B. Boden-Albala, D. E. Kargman, E. Todd, J. Jones, M. C. Paik, R. L. Sacco, and L. Berglund Relation of Apo(a) Size to Carotid Atherosclerosis in an Elderly Multiethnic Population Arterioscler. Thromb. Vasc. Biol., January 1, 2002; 22(1): 141 - 146. [Abstract] [Full Text] [PDF]
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G. R. Warnick, M. Nauck, and N. Rifai Evolution of Methods for Measurement of HDL-Cholesterol: From Ultracentrifugation to Homogeneous Assays Clin. Chem., September 1, 2001; 47(9): 1579 - 1596. [Abstract] [Full Text] [PDF]
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P. Benlian, C. Cansier, G. Hennache, O. Khallouf, P. Bayer, F. Duron, F. Carrat, R. Couderc, O. Chazouilleres, J. Bardet, et al. Comparison of a New Method for the Direct and Simultaneous Assessment of LDL- and HDL-Cholesterol with Ultracentrifugation and Established Methods Clin. Chem., April 1, 2000; 46(4): 493 - 505. [Abstract] [Full Text] [PDF]
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H. Sugiuchi, T. Irie, Y. Uji, T. Ueno, T. Chaen, K. Uekama, and H. Okabe Homogeneous assay for measuring low-density lipoprotein cholesterol in serum with triblock copolymer and {alpha}-cyclodextrin sulfate Clin. Chem., March 1, 1998; 44(3): 522 - 531. [Abstract] [Full Text] [PDF]
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C. H. Tuck, S. Holleran, and L. Berglund Hormonal Regulation of Lipoprotein(a) Levels: Effects of Estrogen Replacement Therapy on Lipoprotein(a) and Acute Phase Reactants in Postmenopausal Women Arterioscler. Thromb. Vasc. Biol., September 1, 1997; 17(9): 1822 - 1829. [Abstract] [Full Text]
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