Clinical Chemistry
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Clinical Chemistry 41: 187-190, 1995;
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Clinical Chemistry, Vol 41, 187-190, Copyright © 1995 by American Association for Clinical Chemistry

Improved PCR amplification/Hhal restriction for unambiguous determination of apolipoprotein E alleles

E Appel, S Eisenberg and J Roitelman
Institute of Lipid and Atherosclerosis Research, Sheba Medical Center, Tel Hashomer, Israel.

We present a modification to the polymerase chain reaction amplification/Hhal restriction isotyping method for human apolipoprotein (apo) E. This method includes a mutagenic forward primer and 5'-end labeling of both primers. These modifications of the original method described by Hixon and Vernier (J Lipid Res 1990;31:545- 8) allow sensitive and unambiguous determination of apoE genotypes.


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