| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Clinical Chemistry, Vol 41, 191-195, Copyright © 1995 by American Association for Clinical Chemistry
U Turpeinen, U Karjalainen and UH Stenman
Helsinki University Central Hospital, Laboratory, Finland.
Using 123 specimens, we compared the concordance of three different methods for determining glycohemoglobin (GHb): the Diamat (Bio-Rad Laboratories), an automated analyzer measuring HbA1c by cation-exchange chromatography; an assay with the IMx analyzer (Abbott Laboratories), based on boronate affinity binding; and an HPLC method measuring HbA1c by cation-exchange chromatography on a PolyCAT A column (PolyLC Inc.). The Pearson's correlation coefficient between PolyCAT A and Diamat was 0.900 +/- 0.038 (mean +/- 2 SD) and between PolyCAT A and IMx, 0.857 +/- 0.042. However, up to twofold differences were seen in some samples. The proportion of GHb was consistently lower with the PolyCAT A method than with the other two assays, apparently because of better separation of HbA1c from nonglycated coeluting forms of Hb. The difference in glycation percentage between the PolyCAT A and Diamat methods is 2-3% over the whole concentration range. These results point to the limitations of Diamat as a reference method to be used to calibrate other methods for determining HbA1c. Further, a switch from one method to another is likely to cause considerable problems in the clinical follow-up of certain patients.
The following articles in journals at HighWire Press have cited this article:
|
|
 |
|
  F. Q. Nuttall Evidence for Independent Heritability of the Glycation Gap (Glycosylation Gap) Fraction of HbA1c in Nondiabetic Twins: Response to Cohen et al. Diabetes Care, April 1, 2007; 30(4): e14 - e14. [Full Text] [PDF]
|
|
|
|
 |
|
 A. Mosca, R. Paleari, A. Made, C. Ferrero, M. Locatelli, and F. Ceriotti Commutability of control materials in glycohemoglobin determinations Clin. Chem., March 1, 1998; 44(3): 632 - 638. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Kobold, J.-O. Jeppsson, T. Dulffer, A. Finke, W. Hoelzel, and K. Miedema Candidate reference methods for hemoglobin A1c based on peptide mapping Clin. Chem., October 1, 1997; 43(10): 1944 - 1951. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Miedema Electrospray Mass Spectrometry for Measurement of Glycohemoglobin Clin. Chem., May 1, 1997; 43(5): 705 - 707. [Full Text] [PDF]
|
|
|
|
|
|
N. B. Roberts, B. N. Green, and M. Morris Potential of electrospray mass spectrometry for quantifying glycohemoglobin Clin. Chem., May 1, 1997; 43(5): 771 - 778. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. A. Doelman, C. W. M. Siebelder, W. A. Nijhof, C. W. Weykamp, J. Janssens, and T. J. Penders Capillary electrophoresis system for hemoglobin A1c determinations evaluated Clin. Chem., April 1, 1997; 43(4): 644 - 648. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Halwachs-Baumann, S. Katzensteiner, W. Schnedl, P. Purstner, T. Pieber, and M. Wilders-Truschnig Comparative evaluation of three assay systems for automated determination of hemoglobin A1c Clin. Chem., March 1, 1997; 43(3): 511 - 517. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
