Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 41: 232-240, 1995;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by McNamara, J. R.
Right arrow Articles by Schaefer, E. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McNamara, J. R.
Right arrow Articles by Schaefer, E. J.

Clinical Chemistry, Vol 41, 232-240, Copyright © 1995 by American Association for Clinical Chemistry

Immunoseparation method for measuring low-density lipoprotein cholesterol directly from serum evaluated

JR McNamara, TG Cole, JH Contois, CA Ferguson, JM Ordovas and EJ Schaefer
USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA.

Low-density lipoprotein (LDL) cholesterol can not be calculated from other lipid measurements when samples are obtained from nonfasting individuals or when triglycerides are > or = 4.0 g/L. We have evaluated a direct LDL cholesterol assay for analyzing 115 fresh serum samples obtained from fasting and nonfasting dyslipidemic patients with triglycerides < or = 35.85 g/L, who were receiving diet and (or) drug treatments. Results were highly correlated with those by ultracentrifugation (r = 0.97), with a mean/median bias of -2.9%/0.7% (- 0.001/0.010 g/L) and an absolute bias of 9.5%/6.4% (0.119/0.090 g/L). The assay correctly classified LDL cholesterol concentrations < 1.30 g/L 81% of the time, 1.30-1.60 g/L 76% of the time, and > or = 1.60 g/L 94% of the time. Precision studies provided within- and between-run CVs in the range of 1.2-3.8% and 2.0-5.1%, respectively. Our data indicate that this assay is an accurate method for measuring LDLC directly from fresh serum obtained from fasting or nonfasting subjects with a wide range of triglyceride values.


The following articles in journals at HighWire Press have cited this article:


Home page
 J CARDIOVASC PHARMACOL THERHome page
E. T. Bairaktari, K. I. Seferiadis, and M. S. Elisaf
Evaluation of Methods for the Measurement of Low-Density Lipoprotein Cholesterol
Journal of Cardiovascular Pharmacology and Therapeutics, January 1, 2005; 10(1): 45 - 54.
[Abstract] [PDF]

Home page
 J Am Coll CardiolHome page
S. M. Grundy, J. I. Cleeman, C. N. Bairey Merz, H. B. Brewer Jr, L. T. Clark, D. B. Hunninghake, R. C. Pasternak, S. C. Smith Jr, N. J. Stone, and Coordinating Committee of the National Cholesterol
Implications of recent clinical trials for the National Cholesterol Education Program Adult Treatment Panel III Guidelines
J. Am. Coll. Cardiol., August 4, 2004; 44(3): 720 - 732.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
S. M. Grundy, J. I. Cleeman, C. N. B. Merz, H. B. Brewer Jr, L. T. Clark, D. B. Hunninghake, R. C. Pasternak, S. C. Smith Jr, N. J. Stone, for the Coordinating Committee of the National Cho, et al.
Implications of Recent Clinical Trials for the National Cholesterol Education Program Adult Treatment Panel III Guidelines
Arterioscler Thromb Vasc Biol, August 1, 2004; 24(8): e149 - e161.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
S. M. Grundy, J. I. Cleeman, C. N. B. Merz, H. B. Brewer Jr, L. T. Clark, D. B. Hunninghake, R. C. Pasternak, S. C. Smith Jr, N. J. Stone, for the Coordinating Committee of the National Cho, et al.
Implications of Recent Clinical Trials for the National Cholesterol Education Program Adult Treatment Panel III Guidelines
Circulation, July 13, 2004; 110(2): 227 - 239.
[Abstract] [Full Text] [PDF]

Home page
 Annals of Clinical & Laboratory ScienceHome page
E. Liberopoulos, G. Miltiadous, M. Hatzivassiliou, N. Ayrton, E. Bairaktari, M. Cariolou, and M. Elisaf
Apolipoprotein E Polymorphism in Northwestern Greece: Frequency and Effect on Lipid Parameters
Ann. Clin. Lab. Sci., July 1, 2004; 34(3): 347 - 354.
[Abstract] [Full Text] [PDF]

Home page
 NEJMHome page
M. E. Brousseau, E. J. Schaefer, M. L. Wolfe, L. T. Bloedon, A. G. Digenio, R. W. Clark, J. P. Mancuso, and D. J. Rader
Effects of an Inhibitor of Cholesteryl Ester Transfer Protein on HDL Cholesterol
N. Engl. J. Med., April 8, 2004; 350(15): 1505 - 1515.
[Abstract] [Full Text] [PDF]

Home page
 Diabetes CareHome page
S. H. Hsia
Non-HDL Cholesterol: Into the Spotlight
Diabetes Care, January 1, 2003; 26(1): 240 - 242.
[Full Text] [PDF]

Home page
 J. Lipid Res.Home page
B. F. Asztalos, K. V. Horvath, J. R. McNamara, P. S. Roheim, J. J. Rubinstein, and E. J. Schaefer
Effects of atorvastatin on the HDL subpopulation profile of coronary heart disease patients
J. Lipid Res., October 1, 2002; 43(10): 1701 - 1707.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
E. J Schaefer
Lipoproteins, nutrition, and heart disease
Am. J. Clinical Nutrition, February 1, 2002; 75(2): 191 - 212.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
M. Nauck, G. R. Warnick, and N. Rifai
Methods for Measurement of LDL-Cholesterol: A Critical Assessment of Direct Measurement by Homogeneous Assays versus Calculation
Clin. Chem., February 1, 2002; 48(2): 236 - 254.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
J. Ordonez-Llanos, A. M. Wagner, R. Bonet-Marques, J. L. Sanchez-Quesada, F. Blanco-Vaca, and F. Gonzalez-Sastre
Which Cholesterol Are We Measuring with the Roche Direct, Homogeneous LDL-C Plus Assay?
Clin. Chem., January 1, 2001; 47(1): 124 - 126.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
B. D. Ragland, R. J. Konrad, C. Chaffin, C. A. Robinson, and R. W. Hardy
Evaluation of a Homogeneous Direct LDL-Cholesterol Assay in Diabetic Patients: Effect of Glycemic Control
Clin. Chem., November 1, 2000; 46(11): 1848 - 1851.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
M. Esteban-Salan, A. Guimon-Bardesi, J. M. de la Viuda-Unzueta, M. N. Azcarate-Ania, P. Pascual-Usandizaga, and E. Amoroto-Del-Rio
Analytical and Clinical Evaluation of Two Homogeneous Assays for LDL-Cholesterol in Hyperlipidemic Patients
Clin. Chem., August 1, 2000; 46(8): 1121 - 1131.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
F. Gomez, J. Camps, J. M. Simo, N. Ferre, and J. Joven
Agreement Study of Methods Based on the Elimination Principle for the Measurement of LDL- and HDL-Cholesterol Compared with Ultracentrifugation in Patients with Liver Cirrhosis
Clin. Chem., August 1, 2000; 46(8): 1188 - 1191.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
P. Benlian, C. Cansier, G. Hennache, O. Khallouf, P. Bayer, F. Duron, F. Carrat, R. Couderc, O. Chazouilleres, J. Bardet, et al.
Comparison of a New Method for the Direct and Simultaneous Assessment of LDL- and HDL-Cholesterol with Ultracentrifugation and Established Methods
Clin. Chem., April 1, 2000; 46(4): 493 - 505.
[Abstract] [Full Text] [PDF]

Home page
 Arch Pediatr Adolesc MedHome page
B. S. Ticho, E. J. Neufeld, J. W. Newburger, N. Harris, A. Baker, and N. Rifai
Utility of Direct Measurement of Low-Density Lipoprotein Cholesterol in Dyslipidemic Pediatric Patients
Arch Pediatr Adolesc Med, August 1, 1998; 152(8): 787 - 791.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
J. R. McNamara, P. K. Shah, K. Nakajima, L. A. Cupples, P. W. F. Wilson, J. M. Ordovas, and E. J. Schaefer
Remnant lipoprotein cholesterol and triglyceride reference ranges from the Framingham Heart Study
Clin. Chem., June 1, 1998; 44(6): 1224 - 1232.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
P. W. F. Wilson, R. B. D'Agostino, D. Levy, A. M. Belanger, H. Silbershatz, and W. B. Kannel
Prediction of Coronary Heart Disease Using Risk Factor Categories
Circulation, May 19, 1998; 97(18): 1837 - 1847.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
J. R. McNamara, E. T. Leary, F. Ceriotti, C. M. Boersma-Cobbaert, T. G. Cole, D. J. Hassemer, M. Nakamura, C. J. Packard, D. W. Seccombe, M. M. Kimberly, et al.
Point Status of lipid and lipoprotein standardization
Clin. Chem., August 1, 1997; 43(8): 1306 - 1310.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
A. Maitra, S. V. Hirany, and I. Jialal
Comparison of two assays for measuring LDL cholesterol
Clin. Chem., June 1, 1997; 43(6): 1040 - 1047.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
T. Planella, M. Cortes, C. Martinez-Bru, F. Gonzalez-Sastre, and J. Ordonez-Llanos
Calculation of LDL-cholesterol by using apolipoprotein B for classification of nonchylomicronemic dyslipemia
Clin. Chem., May 1, 1997; 43(5): 808 - 815.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
L. Vrga, C. Contacos, S. C. H. Li, and D. R. Sullivan
Comparison of methods for measurement of apolipoprotein B and cholesterol in low-density lipoproteins
Clin. Chem., February 1, 1997; 43(2): 390 - 393.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1995 by the American Association for Clinical Chemistry.