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Clinical Chemistry 41: 264-270, 1995;
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Clinical Chemistry, Vol 41, 264-270, Copyright © 1995 by American Association for Clinical Chemistry

Laboratory methods and quality assurance in the Cardiovascular Health Study

M Cushman, ES Cornell, PR Howard, EG Bovill and RP Tracy
Department of Pathology, University of Vermont College of Medicine, Burlington 05401.

The Cardiovascular Health Study is an observational cohort study of risk factors for cardiovascular disease in 5201 participants, ages > or = 65 years. We report the methods and quality-assurance results for blood procurement, processing, shipping, storage, and sample analysis used during the first examination period (May 1989-June 1990). The most frequent difficulty in phlebotomy and processing was the requirement of more than one venipuncture (in 2.6% of the participants). The CVs for control materials ranged from 0.93% for glucose to 10.7% for insulin; most were < 4%. In addition to standard quality-assurance methods, we applied two other methods: technical error calculation for replicates, and weighted linear regression to assess time trend in results of control materials. After outliers were excluded, technical error values ranged from 1.7 for uric acid to 18.8 for insulin. Factor VII and factor VIII had slight trends over the 12-month analysis period. Results of quality-assurance analyses used to resolve problems were successful, thereby improving the second laboratory examination.


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 Arterioscler. Thromb. Vasc. Bio.Home page
N. S. Jenny, R. P. Tracy, M. S. Ogg, L. A. Luong, L. H. Kuller, A. M. Arnold, A. R. Sharrett, and S. E. Humphries
In the Elderly, Interleukin-6 Plasma Levels and the -174G>C Polymorphism Are Associated With the Development of Cardiovascular Disease
Arterioscler Thromb Vasc Biol, December 1, 2002; 22(12): 2066 - 2071.
[Abstract] [Full Text] [PDF]

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 Arch Intern MedHome page
B. M. Psaty, T. A. Manolio, N. L. Smith, S. R. Heckbert, J. S. Gottdiener, G. L. Burke, J. Weissfeld, P. Enright, T. Lumley, N. Powe, et al.
Time Trends in High Blood Pressure Control and the Use of Antihypertensive Medications in Older Adults: The Cardiovascular Health Study
Arch Intern Med, November 11, 2002; 162(20): 2325 - 2332.
[Abstract] [Full Text] [PDF]

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 Arterioscler. Thromb. Vasc. Bio.Home page
A. B. Newman, B. L. Naydeck, K. Sutton-Tyrrell, D. Edmundowicz, D. O'Leary, R. Kronmal, G. L. Burke, and L. H. Kuller
Relationship Between Coronary Artery Calcification and Other Measures of Subclinical Cardiovascular Disease in Older Adults
Arterioscler Thromb Vasc Biol, October 1, 2002; 22(10): 1674 - 1679.
[Abstract] [Full Text] [PDF]

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 Arch Intern MedHome page
A. W. Tsai, M. Cushman, W. D. Rosamond, S. R. Heckbert, J. F. Polak, and A. R. Folsom
Cardiovascular Risk Factors and Venous Thromboembolism Incidence: The Longitudinal Investigation of Thromboembolism Etiology
Arch Intern Med, May 27, 2002; 162(10): 1182 - 1189.
[Abstract] [Full Text] [PDF]

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 Arterioscler. Thromb. Vasc. Bio.Home page
A. B. Newman, B. L. Naydeck, J. Whittle, K. Sutton-Tyrrell, D. Edmundowicz, and L. H. Kuller
Racial Differences in Coronary Artery Calcification in Older Adults
Arterioscler Thromb Vasc Biol, March 1, 2002; 22(3): 424 - 430.
[Abstract] [Full Text] [PDF]

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 Arch Intern MedHome page
N. L. Smith, J. I. Barzilay, D. Shaffer, P. J. Savage, S. R. Heckbert, L. H. Kuller, R. A. Kronmal, H. E. Resnick, and B. M. Psaty
Fasting and 2-Hour Postchallenge Serum Glucose Measures and Risk of Incident Cardiovascular Events in the Elderly: The Cardiovascular Health Study
Arch Intern Med, January 28, 2002; 162(2): 209 - 216.
[Abstract] [Full Text] [PDF]

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 CirculationHome page
A. B. Newman, B. L. Naydeck, K. Sutton-Tyrrell, A. Feldman, D. Edmundowicz, and L. H. Kuller
Coronary Artery Calcification in Older Adults to Age 99: Prevalence and Risk Factors
Circulation, November 27, 2001; 104(22): 2679 - 2684.
[Abstract] [Full Text] [PDF]

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 DiabetesHome page
J. I. Barzilay, L. Abraham, S. R. Heckbert, M. Cushman, L. H. Kuller, H. E. Resnick, and R. P. Tracy
The Relation of Markers of Inflammation to the Development of Glucose Disorders in the Elderly: The Cardiovascular Health Study
Diabetes, October 1, 2001; 50(10): 2384 - 2389.
[Abstract] [Full Text]

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 Diabetes CareHome page
J. I. Barzilay, C. F. Spiekerman, L. H. Kuller, G. L. Burke, V. Bittner, J. S. Gottdiener, F. L. Brancati, T. J. Orchard, D. H. O'Leary, and P. J. Savage
Prevalence of Clinical and Isolated Subclinical Cardiovascular Disease in Older Adults With Glucose Disorders: The Cardiovascular Health Study
Diabetes Care, July 1, 2001; 24(7): 1233 - 1239.
[Abstract] [Full Text] [PDF]

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 Arch Intern MedHome page
B. M. Psaty, C. D. Furberg, L. H. Kuller, M. Cushman, P. J. Savage, D. Levine, D. H. O'Leary, R. N. Bryan, M. Anderson, and T. Lumley
Association Between Blood Pressure Level and the Risk of Myocardial Infarction, Stroke, and Total Mortality: The Cardiovascular Health Study
Arch Intern Med, May 14, 2001; 161(9): 1183 - 1192.
[Abstract] [Full Text] [PDF]

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 Am J EpidemiolHome page
D. F. Geffken, M. Cushman, G. L. Burke, J. F. Polak, P. A. Sakkinen, and R. P. Tracy
Association between Physical Activity and Markers of Inflammation in a Healthy Elderly Population
Am. J. Epidemiol., February 1, 2001; 153(3): 242 - 250.
[Abstract] [Full Text] [PDF]

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 Am J EpidemiolHome page
P. A. Sakkinen, P. Wahl, M. Cushman, M. R. Lewis, and R. P. Tracy
Clustering of Procoagulation, Inflammation, and Fibrinolysis Variables with Metabolic Factors in Insulin Resistance Syndrome
Am. J. Epidemiol., November 15, 2000; 152(10): 897 - 907.
[Abstract] [Full Text] [PDF]

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 CirculationHome page
D. S. Siscovick, S. M. Schwartz, L. Corey, J. T. Grayston, R. Ashley, S.-P. Wang, B. M. Psaty, R. P. Tracy, L. H. Kuller, and R. A. Kronmal
Chlamydia pneumoniae, Herpes Simplex Virus Type 1, and Cytomegalovirus and Incident Myocardial Infarction and Coronary Heart Disease Death in Older Adults : The Cardiovascular Health Study
Circulation, November 7, 2000; 102(19): 2335 - 2340.
[Abstract] [Full Text] [PDF]

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 Arterioscler. Thromb. Vasc. Bio.Home page
L. H. Kuller, P. Velentgas, J. Barzilay, N. J. Beauchamp, D. H. O’Leary, and P. J. Savage
Diabetes Mellitus : Subclinical Cardiovascular Disease and Risk of Incident Cardiovascular Disease and All-Cause Mortality
Arterioscler Thromb Vasc Biol, March 1, 2000; 20(3): 823 - 829.
[Abstract] [Full Text] [PDF]

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 JNCI J Natl Cancer InstHome page
R. E. Schoen, C. M. Tangen, L. H. Kuller, G. L. Burke, M. Cushman, R. P. Tracy, A. Dobs, and P. J. Savage
Increased Blood Glucose and Insulin, Body Size, and Incident Colorectal Cancer
J Natl Cancer Inst, July 7, 1999; 91(13): 1147 - 1154.
[Abstract] [Full Text] [PDF]

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 Arterioscler. Thromb. Vasc. Bio.Home page
R. P. Tracy, A. M. Arnold, W. Ettinger, L. Fried, E. Meilahn, and P. Savage
The Relationship of Fibrinogen and Factors VII and VIII to Incident Cardiovascular Disease and Death in the Elderly : Results From the Cardiovascular Health Study
Arterioscler Thromb Vasc Biol, July 1, 1999; 19(7): 1776 - 1783.
[Abstract] [Full Text] [PDF]

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 Arch Intern MedHome page
B. M. Psaty, C. D. Furberg, L. H. Kuller, D. E. Bild, P. M. Rautaharju, J. F. Polak, E. Bovill, and J. S. Gottdiener
Traditional Risk Factors and Subclinical Disease Measures as Predictors of First Myocardial Infarction in Older Adults: The Cardiovascular Health Study
Arch Intern Med, June 28, 1999; 159(12): 1339 - 1347.
[Abstract] [Full Text] [PDF]

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 Arterioscler. Thromb. Vasc. Bio.Home page
P. A. Sakkinen, M. Cushman, B. M. Psaty, B. Rodriguez, R. Boineau, L. H. Kuller, and R. P. Tracy
Relationship of Plasmin Generation to Cardiovascular Disease Risk Factors in Elderly Men and Women
Arterioscler Thromb Vasc Biol, March 1, 1999; 19(3): 499 - 504.
[Abstract] [Full Text] [PDF]

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 CirculationHome page
M. A. Espeland, S. M. Marcovina, V. Miller, P. D. Wood, C. Wasilauskas, R. Sherwin, H. Schrott, and T. L. Bush
Effect of Postmenopausal Hormone Therapy on Lipoprotein(a) Concentration
Circulation, March 17, 1998; 97(10): 979 - 986.
[Abstract] [Full Text] [PDF]

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 Clin. Chem.Home page
R. S. Rosenson, B. A. Staffileno, and C. C. Tangney
Effects of Tourniquet Technique, Order of Draw, and Sample Storage on Plasma Fibrinogen
Clin. Chem., March 1, 1998; 44(3): 688 - 690.
[Full Text] [PDF]

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 JAMAHome page
L. P. Fried, R. A. Kronmal, A. B. Newman, D. E. Bild, M. B. Mittelmark, J. F. Polak, J. A. Robbins, J. M. Gardin, and for the Cardiovascular Health Study Collaborative
Risk Factors for 5-Year Mortality in Older Adults: The Cardiovascular Health Study
JAMA, February 25, 1998; 279(8): 585 - 592.
[Abstract] [Full Text] [PDF]

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 CirculationHome page
B. M. Psaty, T. A. Manolio, L. H. Kuller, R. A. Kronmal, M. Cushman, L. P. Fried, R. White, C. D. Furberg, and P. M. Rautaharju
Incidence of and Risk Factors for Atrial Fibrillation in Older Adults
Circulation, October 7, 1997; 96(7): 2455 - 2461.
[Abstract] [Full Text]

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 Arterioscler. Thromb. Vasc. Bio.Home page
R. P. Tracy, B. M. Psaty, E. Macy, E. G. Bovill, M. Cushman, E. S. Cornell, and L. H. Kuller
Lifetime Smoking Exposure Affects the Association of C-Reactive Protein with Cardiovascular Disease Risk Factors and Subclinical Disease in Healthy Elderly Subjects
Arterioscler Thromb Vasc Biol, October 1, 1997; 17(10): 2167 - 2176.
[Abstract] [Full Text]

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 Arterioscler. Thromb. Vasc. Bio.Home page
R. P. Tracy, R. N. Lemaitre, B. M. Psaty, D. G. Ives, R. W. Evans, M. Cushman, E. N. Meilahn, and L. H. Kuller
Relationship of C-Reactive Protein to Risk of Cardiovascular Disease in the Elderly: Results From the Cardiovascular Health Study and the Rural Health Promotion Project
Arterioscler Thromb Vasc Biol, June 1, 1997; 17(6): 1121 - 1127.
[Abstract] [Full Text]

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 Arterioscler. Thromb. Vasc. Bio.Home page
H. G. Alcorn, S. K. Wolfson Jr, K. Sutton-Tyrrell, L. H. Kuller, D. O'Leary, and
Risk Factors for Abdominal Aortic Aneurysms in Older Adults Enrolled in the Cardiovascular Health Study
Arterioscler Thromb Vasc Biol, August 1, 1996; 16(8): 963 - 970.
[Abstract] [Full Text]

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 Arterioscler. Thromb. Vasc. Bio.Home page
D. S. Sharp, R. D. Abbott, C. M. Burchfiel, B. L. Rodriguez, R. P. Tracy, K. Yano, and J. D. Curb
Plasma Fibrinogen and Coronary Heart Disease in Elderly Japanese-American Men
Arterioscler Thromb Vasc Biol, February 1, 1996; 16(2): 262 - 268.
[Abstract] [Full Text]

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 Arterioscler. Thromb. Vasc. Bio.Home page
R. P. Tracy, E. G. Bovill, D. Yanez, B. M. Psaty, L. P. Fried, G. Heiss, M. Lee, J. F. Polak, and P. J. Savage
Fibrinogen and Factor VIII, but Not Factor VII, Are Associated With Measures of Subclinical Cardiovascular Disease in the Elderly : Results From the Cardiovascular Health Study
Arterioscler Thromb Vasc Biol, September 1, 1995; 15(9): 1269 - 1279.
[Abstract] [Full Text]


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