Clinical Chemistry
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Clinical Chemistry 41: 300-305, 1995;
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Clinical Chemistry, Vol 41, 300-305, Copyright © 1995 by American Association for Clinical Chemistry

Simultaneous determination of alcohols and ethylene glycol in serum by packed- or capillary-column gas chromatography

JF Livesey, SL Perkins, NE Tokessy and MJ Maddock
Department of Laboratory Medicine, Ottawa Civic Hospital, Ontario, Canada.

We developed a packed-column chromatographic procedure capable of simultaneous quantitation of methanol, ethanol, isopropanol, acetone, and ethylene glycol. This method was then updated to a rapid, sensitive, wide-bore capillary method. The packed-column system uses direct injection of 1 microL of Na2WO4/H2SO4-deproteinized serum onto a 1.8 m x 2 mm (i.d.) column packed with 80/100 HayeSep R. A linear temperature gradient from 90 to 205 degrees C allows complete elution of all components within 20 min; minimum detection limits are 2 mmol/L. The wide-bore capillary method uses 0.1 microL of sample deproteinized by ultrafiltration, injected onto a 30 m x 0.53 mm (i.d.) 3-microns Rtx- 200 (Restek) column. Baseline resolution to a minimum detection limit of 0.1 mmol/L of all compounds is achieved in 5 min with a linear temperature gradient from 40 to 250 degrees C and dual internal standards of n-propanol and 1,2-butanediol.


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