| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Clinical Chemistry, Vol 41, 361-366, Copyright © 1995 by American Association for Clinical Chemistry
A Andersson, A Lindgren and B Hultberg
Department of Clinical Chemistry, University Hospital, Lund, Sweden.
Changes in concentration of reduced and oxidized low-M(r) thiols were measured in blood and plasma before and after the separation of blood cells. If centrifugation of blood was postponed, the reduced form of homocysteine in plasma increased with time at 22 degrees C; in contrast, the concentrations of other reduced thiols (cysteine, glutathione, and cysteinylglycine) decreased. In plasma the reduced forms of all thiols disappeared at a rate that followed first-order kinetics. The rates of disappearance ("half-lives") were temperature- dependent; they were about the same for glutathione and homocysteine (11.7 and 14.3 min, respectively, at 22 degrees C) and somewhat higher for cysteinylglycine and cysteine. After establishing proper sampling conditions for reduced thiols, we measured this thiol fraction as well as free (non-protein-bound) and total thiols in 10 reference subjects and 19 patients with cerebral infarction. Mild but significant hyperhomocysteinemia involving total and free homocysteine (but not reduced homocysteine) was found in the patients.
The following articles in journals at HighWire Press have cited this article:
|
|
 |
|
  K. S. McCully Chemical Pathology of Homocysteine. IV. Excitotoxicity, Oxidative Stress, Endothelial Dysfunction, and Inflammation Ann. Clin. Lab. Sci., January 1, 2009; 39(3): 219 - 232. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Antoniades, A. S. Antonopoulos, D. Tousoulis, K. Marinou, and C. Stefanadis Homocysteine and coronary atherosclerosis: from folate fortification to the recent clinical trials Eur. Heart J., January 1, 2009; 30(1): 6 - 15. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. V. Mohan, I. A. Jagroop, D. P. Mikhailidis, and G. P. Stansby Homocysteine Activates Platelets In Vitro Clinical and Applied Thrombosis/Hemostasis, January 1, 2008; 14(1): 8 - 18. [Abstract] [PDF]
|
|
|
|
 |
|
 A. B. Sobol, E. Bald, and J. Loba Fractions of Total Plasma Homocysteine in Patients with Ischemic Stroke Before the Age of 55 Years Angiology, March 1, 2005; 56(2): 201 - 209. [Abstract] [PDF]
|
|
|
|
|
|
P. Lind, B. Hedblad, B. Hultberg, L. Stavenow, L. Janzon, and F. Lindgarde Risk of Myocardial Infarction in Relation to Plasma Levels of Homocysteine and Inflammation-Sensitive Proteins: A Long-Term Nested Case-Control Study Angiology, July 1, 2003; 54(4): 401 - 410. [Abstract] [PDF]
|
|
|
|
 |
|
 H. Sato, M. Tamba, S. Okuno, K. Sato, K. Keino-Masu, M. Masu, and S. Bannai Distribution of Cystine/Glutamate Exchange Transporter, System xc-, in the Mouse Brain J. Neurosci., September 15, 2002; 22(18): 8028 - 8033. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Weiss, C. Keller, U. Hoffmann, and J. Loscalzo Endothelial dysfunction and atherothrombosis in mild hyperhomocysteinemia Vascular Medicine, August 1, 2002; 7(3): 227 - 239. [Abstract] [PDF]
|
|
|
|
 |
|
 R. H. Williams, J. A. Maggiore, R. D. Reynolds, and C. M. Helgason Novel Approach for the Determination of the Redox Status of Homocysteine and Other Aminothiols in Plasma from Healthy Subjects and Patients with Ischemic Stroke Clin. Chem., June 1, 2001; 47(6): 1031 - 1039. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Herrmann, H. Schorr, K. Purschwitz, F. Rassoul, and V. Richter Total Homocysteine, Vitamin B12, and Total Antioxidant Status in Vegetarians Clin. Chem., June 1, 2001; 47(6): 1094 - 1101. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Andersson, A. Lindgren, M. Arnadottir, H. Prytz, and B. Hultberg Thiols as a Measure of Plasma Redox Status in Healthy Subjects and in Patients with Renal or Liver Failure Clin. Chem., July 1, 1999; 45(7): 1084 - 1087. [Full Text] [PDF]
|
|
|
|
|
|
A. Gardemann, H. Weidemann, M. Philipp, N. Katz, H. Tillmanns, F. W. Hehrlein, and W. Haberbosch The TT genotype of the methylenetetrahydrofolate reductase C677T gene polymorphism is associated with the extent of coronary atherosclerosis in patients at high risk for coronary artery disease Eur. Heart J., April 2, 1999; 20(8): 584 - 592. [Abstract] [PDF]
|
|
|
|
 |
|
 G. N. Welch and J. Loscalzo Homocysteine and Atherothrombosis N. Engl. J. Med., April 9, 1998; 338(15): 1042 - 1050. [Full Text] [PDF]
|
|
|
|
 |
|
 G. R. Upchurch Jr., G. N. Welch, A. J. Fabian, J. E. Freedman, J. L. Johnson, J. F. Keaney Jr., and J. Loscalzo Homocyst(e)ine Decreases Bioavailable Nitric Oxide by a Mechanism Involving Glutathione Peroxidase J. Biol. Chem., July 4, 1997; 272(27): 17012 - 17017. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sengupta, H. Chen, T. Togawa, P. M. DiBello, A. K. Majors, B. Budy, M. E. Ketterer, and D. W. Jacobsen Albumin Thiolate Anion Is an Intermediate in the Formation of Albumin-S-S-Homocysteine J. Biol. Chem., August 3, 2001; 276(32): 30111 - 30117. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
