Clinical Chemistry
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Clinical Chemistry 41: 392-396, 1995;
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Clinical Chemistry, Vol 41, 392-396, Copyright © 1995 by American Association for Clinical Chemistry

Effect of storage at 4 degrees C and -20 degrees C on lipid, lipoprotein, and apolipoprotein concentrations

K Evans, J Mitcheson and MF Laker
Department of Clinical Biochemistry and Metabolic Medicine, University of Newcastle upon Tyne, Medical School, UK.

We have investigated the effects on lipid, apolipoprotein, and lipoprotein measurements of storing unfractionated serum at 4 degrees C for 10 days and at -20 degrees C for 10 days or 3 months. Total serum concentrations of lipids were stable, although apolipoprotein B showed a 5.3% increase after 3 months at -20 degrees C (P < 0.001). Increases in low-density (LDL) and high-density lipoprotein (HDL) triglyceride and very-low-density lipoprotein (VLDL) esterified cholesterol concentrations and decreases in free cholesterol concentrations in LDL and HDL after storage of serum for 10 days at 4 degrees C were verified by fractionation of lipoproteins by sequential flotation ultracentrifugation. Ten days' storage of serum at -20 degrees C resulted in increases in VLDL triglyceride and phospholipid concentrations, with decreases in HDL concentrations in triglycerides and phospholipids; changes were more extensive after 3 months at -20 degrees C. We conclude that ultracentrifugation of serum for lipoprotein analysis should be performed as soon as possible after collection.


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R. Cramb, J. French, M. Mackness, R D. G Neely, M. Caslake, and F. MacKenzie
Lipid external quality assessment: commutability between external quality assessment and clinical specimens
Ann Clin Biochem, May 1, 2008; 45(3): 260 - 265.
[Abstract] [Full Text] [PDF]


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