Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 41: 458-461, 1995;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Speakman, E. D.
Right arrow Articles by Bruns, D. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Speakman, E. D.
Right arrow Articles by Bruns, D. E.

Clinical Chemistry, Vol 41, 458-461, Copyright © 1995 by American Association for Clinical Chemistry

Measurement of methemoglobin in neonatal samples containing fetal hemoglobin

ED Speakman, JC Boyd and DE Bruns
Department of Pathology, University of Virginia Health Sciences Center, Charlottesville 22908.

Because of the potential for methemoglobinemia during nitric oxide therapy in newborns, methods are needed to accurately quantify methemoglobin (MetHb) in the presence of the high concentrations of fetal hemoglobin (Hb F), bilirubin, and lipids seen in these patients. Spectral differences between fetal and adult Hbs invalidate assumptions of conventional multiwavelength Hb photometry, so we evaluated an "overdetermined" system (Ciba-Corning Model 270), in which absorbances at seven wavelengths are measured to quantify four Hb derivatives. Adult and umbilical cord blood (Hb F 96%) samples were prepared to contain known MetHb fractions. Measured MetHb was linear in cord blood to > or = 15% MetHb. Within-run precision (CV) was < 2.2% (n = 10) at each of seven MetHb fractions between 5% and 100%. Measured (y) and expected (x) MetHb fractions in cord blood were in good agreement (y = 1.0200x + 0.100, Sylx = 0). Added bilibrubin (200 mg/L serum) and lipid (30 g/L) did not interfere. No significant differences were seen for adult and cord blood samples with identical MetHb fractions (P = 0.72), whereas a significant difference was noted with an exactly determined system (P = 0.0033). At clinically relevant MetHb fractions (< 15%), a trend towards increased values in cord blood was noted with an exactly determined system (y = 1.0520x + 0.7600). We conclude that this overdetermined system measures MetHb accurately in samples from patients with large concentrations of Hb F.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
P. L. M. Lynch and M. J. O'Kane
Methemoglobin Interferes with the HemoCue B-Glucose Analyzer
Clin. Chem., March 1, 2002; 48(3): 581 - 583.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
M. H. de Keijzer, R. Brandts, and B. A.J. Giesendorf
Comment on the Overestimation of Methemoglobin Concentrations in Neonatal Samples with the Chiron 800 System CO-Oximeter Module
Clin. Chem., August 1, 1999; 45(8): 1313 - 1314.
[Full Text]

Home page
 Clin. Chem.Home page
P. L. M. Lynch, D. E. Bruns, J. C. Boyd, and J. Savory
Chiron 800 System CO-oximeter Module Overestimates Methemoglobin Concentrations in Neonatal Samples Containing Fetal Hemoglobin
Clin. Chem., July 1, 1998; 44(7): 1569 - 1570.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1995 by the American Association for Clinical Chemistry.