Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 41: 564-570, 1995;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Luppa, P.
Right arrow Articles by Hauptmann, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Luppa, P.
Right arrow Articles by Hauptmann, H.

Clinical Chemistry, Vol 41, 564-570, Copyright © 1995 by American Association for Clinical Chemistry

6 alpha-biotinylated estrone: novel tracer in competitive chemiluminescence immunoassay of estrone in serum

P Luppa, S Hauck, I Schwab, C Birkmayer and H Hauptmann
Institute of Clinical Chemistry and Pathobiochemistry, Klinikum rechts der Isar, Technical University Munich, Germany.

We describe the development and validation of a labeled-hapten competitive immunoassay for determining total estrone in serum. For the hapten tracer we use the 6 alpha-biotinylated estrone derivative, 3- hydroxyestra-1,3,5(10)-trien-17-one 6 alpha-N-(epsilon- biotinyl)aminocaproamide (Bio-E1). A specific polyclonal rabbit anti- estrone antibody is indirectly bound via an immobilized donkey anti- rabbit antibody on microtiter plate wells. The amount of Bio-E1 bound is then measured with streptavidin-horseradish peroxidase conjugate, whereby the enzyme activity is quantified by an enhanced chemiluminometric method. For the assay, serum samples were extracted with solid-phase extraction cartridges. The assay dynamic range was 93- 7400 pmol/L estrone, with a lower detection limit of 55 pmol/L. An interassay imprecision (CV) of 12-14%, a recovery rate between 80% and 110%, and a dilution linearity are demonstrated. Estrone serum concentrations were measured in healthy men and women and in women with polycystic ovary syndrome. Comparing the assay with a nonextraction RIA, we found an acceptable correlation for samples from 143 subjects of either sex. This enzyme immunoassay with biotin as the primary label and enhanced chemiluminescence signaling detection performs well for determining total estrone in serum and is readily adaptable to assays for other steroid hormones.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
P. Luppa, C. Bruckner, I. Schwab, S. Hauck, S. Schmidmayr, C. Birkmayer, B. Paulus, and H. Hauptmann
7{alpha}-Biotinylated testosterone derivatives as tracers for a competitive chemiluminescence immunoassay of testosterone in serum
Clin. Chem., December 1, 1997; 43(12): 2345 - 2352.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1995 by the American Association for Clinical Chemistry.