Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 41: 914-919, 1995;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lagrost, L.
Right arrow Articles by Lallemant, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lagrost, L.
Right arrow Articles by Lallemant, C.

Clinical Chemistry, Vol 41, 914-919, Copyright © 1995 by American Association for Clinical Chemistry

Immunospecific scintillation proximity assay of cholesteryl ester transfer protein activity

L Lagrost, N Loreau, P Gambert and C Lallemant
Laboratoire de Biochimie des Lipoproteines, INSERM CJF 93-10, Faculte de Medecine, Dijon, France.

We describe a novel, immunospecific scintillation proximity assay for determining cholesteryl ester transfer protein (CETP) activity in total human serum and in reconstituted experimental mixtures. The assay is based on the measurement of radiolabeled cholesteryl esters transferred from a tracer dose of biosynthetically labeled high-density lipoprotein subfraction 3 to unlabeled apolipoprotein (apo) B-containing lipoproteins. The radioactivity content of the apo B-containing lipoprotein fraction can be evaluated without separating the donor from the acceptor lipoprotein substrates, and is measured through the formation of ternary complexes involving the radiolabeled apo B- containing lipoproteins, specific anti-apo B antibodies from sheep, and anti-sheep antibody-labeled fluoromicrospheres. Good correspondences were observed between CETP activity values obtained either with the ultracentrifugation method or the immunospecific scintillation proximity assay (n = 70; r = 0.94; P = 0.0001), and between values obtained for either fresh or frozen serum samples (n = 70; r = 0.93; P = 0.0001). Because of its potential for automation, the immunospecific scintillation proximity assay may constitute a convenient tool to measure serum CETP activity in the clinical laboratory.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1995 by the American Association for Clinical Chemistry.