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Clinical Chemistry 41: 1028-1032, 1995;
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Clinical Chemistry, Vol 41, 1028-1032, Copyright © 1995 by American Association for Clinical Chemistry

Protocol for collection and HPLC analysis of volatile carbonyl compounds in breath

Y Lin, SR Dueker, AD Jones, SE Ebeler and AJ Clifford
Department of Nutrition, University of California, Davis 95616, USA.

This noninvasive method for collection and analysis of a wide range of aldehydes and ketones in human breath may enable assessment of lipid peroxidation and metabolic status in vivo. Breath samples are drawn through silica cartridges impregnated with 2,4-dinitrophenylhydrazine, which traps carbonyls as their hydrazone derivatives. The hydrazone derivatives are eluted from the cartridges with acetonitrile, separated by reversed-phase HPLC, and quantified spectrophotometrically. Using this method, we have measured formaldehyde, acetaldehyde, acetone, propanal, 2-butanone, butanal, pentanal, and hexanal. Recoveries of carbonyls added to Douglas bags were > 90%, except for 2-butanone, which was 86.2%. The overall CVs for sampling plus analyzing duplicate aliquots of breath were < 11%. The results indicate that this protocol can be used to monitor changes of carbonyl production by analyzing expired air, which may, with further study, indicate physiological and pathological status.


The following articles in journals at HighWire Press have cited this article:


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D. Smith, P. Spanel, and S. Davies
Trace gases in breath of healthy volunteers when fasting and after a protein-calorie meal: a preliminary study
J Appl Physiol, November 1, 1999; 87(5): 1584 - 1588.
[Abstract] [Full Text] [PDF]


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Copyright © 1995 by the American Association for Clinical Chemistry.