Clinical Chemistry
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Clinical Chemistry 41: 1297-1301, 1995;
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Clinical Chemistry, Vol 41, 1297-1301, Copyright © 1995 by American Association for Clinical Chemistry

Measurement of urinary oxalate and citrate by capillary electrophoresis and indirect ultraviolet absorbance

RP Holmes
Department of Urology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157, USA.

We describe a method for measuring urinary oxalate and citrate with capillary electrophoresis (CE) and indirect ultraviolet absorbance detection. Sample preparation is minimal, requiring an acidification, brief centrifugation, and dilution. The method is rapid, with oxalate and citrate having mean migration times of 4.02 and 4.50 min, respectively. The minimal detectable concentration (signal-to-noise ratio of 7) of both oxalate and citrate in urine was 7 mg/L. Total imprecisions (CV) were 1.2-5.6% for three urine samples with oxalate and citrate concentrations of 8-60 mg/L and 80-860 mg/L, respectively. The recovery of added oxalate ranged from 94% to 101%. Results of CE analyses agreed well with enzymatic determinations of oxalate and citrate. Rapid analysis time, accuracy, and reproducibility make this procedure well suited for routine urinary oxalate and citrate determinations.




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Copyright © 1995 by the American Association for Clinical Chemistry.