Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 41: 1403-1406, 1995;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schmalzing, D.
Right arrow Articles by Fuchs, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schmalzing, D.
Right arrow Articles by Fuchs, M.

Clinical Chemistry, Vol 41, 1403-1406, Copyright © 1995 by American Association for Clinical Chemistry

Solution-phase immunoassay for determination of cortisol in serum by capillary electrophoresis

D Schmalzing, W Nashabeh and M Fuchs
PerSeptive Biosystems, Framingham, MA 01701, USA.

We describe a competitive solution-phase immunoassay for serum cortisol determination that involves capillary electrophoresis (CE) combined with laser-induced fluorescence for separation and quantification. A polyclonal antibody preparation and fluorescein-labeled cortisol are used as assay reagents. 8-Anilino-1-naphthalenesulfonic acid was added to the serum sample during the assay to promote the release of cortisol from endogenous binding proteins. Conditions for the rapid separation of free and bound labeled antigen by CE were developed. Aspects of assay performance are evaluated in this report. The resulting assay protocol allows for the analysis of serum samples without extraction or other sample preparation steps.


The following articles in journals at HighWire Press have cited this article:


Home page
 Am. J. Clin. Nutr.Home page
M. Roberts, W. Geiger, and J B. German
The revolution in microanalytic chemistry: a macro-opportunity for clinical nutrition1
Am. J. Clinical Nutrition, February 1, 2000; 71(2): 434 - 437.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1995 by the American Association for Clinical Chemistry.