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Clinical Chemistry 42: 57-63, 1996;
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Clinical Chemistry, Vol 42, 57-63, Copyright © 1996 by American Association for Clinical Chemistry

Identification and quantification of hemoglobins A, F, S, and C by automated chromatography

C Papadea and JC Cate 4th
Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston 29425, USA. christine_papadea@smtpgw.musc.edu

The Bio-Rad Variant Hemoglobin Testing System is an automated HPLC analyzer marketed with a Beta-thalassemia Short Program to quantify hemoglobins (Hbs) F and A2 and assist in detecting Hbs A, S, C, D, and E. We evaluated this system to replace several traditional methods for Hb in our hospital laboratory. Analytical performance relevant to quantifying Hbs A, S, C, and F was assessed with blood samples obtained from our local patient population. Studies of precision (CVs < 3%) and analytical limits (% of total Hb) of Hbs A (2-86%), F (1-89%), S (5- 90%), and C (3-92%) demonstrated results comparable with or exceeding those of traditional methods. Results for patients' samples (n) for Hbs A (107), F (157), S (128), and C (27) correlated well (r > 0.93) with results by traditional methods. The satisfactory performance and efficiency led us to implement this system for routine quantification of clinically significant Hbs.


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