Clinical Chemistry
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Clinical Chemistry 42: 1639-1644, 1996;
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Clinical Chemistry, Vol 42, 1639-1644, Copyright © 1996 by American Association for Clinical Chemistry

Coated-tube radioimmunoassay for C-telopeptides of type I collagen to assess bone resorption

M Bonde, C Fledelius, P Qvist and C Christiansen
Osteometer BioTech A/S, Herlev, Denmark.

We present a coated-tube RIA that is useful for assessment of bone resorption. The assay uses a monoclonal antibody raised against a linear 8-amino-acid sequence (EKAHDGGR) derived from the C-telopeptides of type I collagen. Within-run and total CVs were 4.4% and 5.3-6.2%, respectively, at concentrations of 1-7 mg/L (n = 4-20). Analytical recovery was 98% +/- 8% and dilution 97% +/- 7%. Values obtained in a group of 36 premenopausal women were 227 +/- 89.6 mg/mol creatinine. In a group of 141 postmenopausal women, the values obtained were 429 +/- 225 mg/mol creatinine, a highly significant increase of 89% (P <0.001) over the premenopausal value. In a double-blind placebo-controlled clinical study of these postmenopausal women receiving five different doses of a bisphosphonate, a significant decrease of RIA-measured C- telopeptide values was seen in all bisphosphonate-treated groups, after just 3 months. Values in urine samples from postmenopausal women assayed with the RIA (gamma) and the CrossLaps(TM) ELISA (x) agreed well: slope = 0.98 (95% confidence interval, 0.94-1.01), intercept = 0.34 (0.25-0.43) mg/L, and Sylx = 0.93 mg/L (n = 678). We conclude that this RIA represents a valuable tool for assessing bone resorption.


The following articles in journals at HighWire Press have cited this article:


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 J. Clin. Endocrinol. Metab.Home page
E. A. Amama, M. Taga, and H. Minaguchi
The Effect of Gonadotropin-Releasing Hormone Agonist on Type I Collagen C-Telopeptide and N-Telopeptide: the Predictive Value of Biochemical Markers of Bone Turnover
J. Clin. Endocrinol. Metab., February 1, 1998; 83(2): 333 - 338.
[Abstract] [Full Text]

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 J. Biol. Chem.Home page
C. Fledelius, A. H. Johnsen, P. A.C. Cloos, M. Bonde, and P. Qvist
Characterization of Urinary Degradation Products Derived from Type I Collagen. IDENTIFICATION OF A beta -ISOMERIZED ASP-GLY SEQUENCE WITHIN THE C-TERMINAL TELOPEPTIDE (alpha 1) REGION
J. Biol. Chem., April 11, 1997; 272(15): 9755 - 9763.
[Abstract] [Full Text] [PDF]


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